Korean J Parasitol.  2014 Dec;52(6):701-705. 10.3347/kjp.2014.52.6.701.

Molecular Characterization of Gastrothylax crumenifer (Platyhelminthes: Gastrothylacidae) from Goats in the Western Part of India by LSU of Nuclear Ribosomal DNA

• Kumar A
• Chaudhary
• Verma C
• Singh HS

• KMID: 2069825
• DOI: http://doi.org/10.3347/kjp.2014.52.6.701

Abstract

The rumen parasite, Gastrothylax crumenifer (Platyhelminthes: Gastrothylacidae), is a highly pathogenic trematode parasite of goat (Capra hircus). It sucks blood that causes acute disease like anemia, and severe economic losses occur due to morbidity and mortality of the ruminant infected by these worms. The study of these rumen paramphistomes, their infection, and public health importance remains unclear in India especially in the western part of state Uttar Pradesh (U.P.), Meerut, India, where the goat meat consumption is very high. This paper provides the molecular characterization of G. crumenifer recovered from the rumen of Capra hircus from Meerut, U.P., India by the partial sequence of 28S rDNA. Nucleotide sequence similarity searching on BLAST of 28S rDNA from parasites showed the highest identity with those of G. crumenifer from the same host Capra hircus. This is the first report of molecular identification of G. crumenifer from this part of India.

Keyword

Gastrothylax crumenifer; goat (Capra hircus); ribosomal DNA; Uttar Pradesh; India

MeSH Terms

Animals
Cluster Analysis
DNA, Helminth/chemistry/genetics
DNA, Ribosomal/chemistry/genetics
Goat Diseases/*parasitology
Goats
India
Microscopy, Electron, Scanning
Molecular Sequence Data
Phylogeny
Platyhelminths/*classification/genetics/*isolation & purification/ultrastructure
RNA, Ribosomal, 28S/genetics
Rumen/parasitology
Sequence Analysis, DNA
Trematode Infections/parasitology/*veterinary
DNA, Helminth
DNA, Ribosomal
RNA, Ribosomal, 28S