Korean J Parasitol.  1997 Dec;35(4):271-276. 10.3347/kjp.1997.35.4.271.

PCR-RFLP pattern of three kinds of Metagonimus in Korea

• Yu JR
• Chung JS
• Huh S
• Lee SH
• Chai JY

• KMID: 1715314
• DOI: http://doi.org/10.3347/kjp.1997.35.4.271

Abstract

We tried to compare the three kinds of Metagonimus species, M. yokogawai, Metagonimus Miyata type, and M. takahashii, which were known to be distributed in Korea with polymerase chain reaction based-restriction fragment length polymorphism (PCR-RFLP) patterns. We amplified the internal transcribed spacer 1 (ITS1) site of ribosomal RNA and mitochondrial cytochrome c oxidase I (mCOI) gene. The restriction patterns of ITS1 gene with Rsa I, Alu I and Msp I showed multiple fragmented bands of different sizes between three kinds of Metagonimus. In case of mCOI gene, Rsa I and Alu I enzymes produced differentially fragmented band patterns. According to the parsimony analysis of PCR-RFLP patterns, the estimated genetic divergence between M. yokogawai and Metagonimus Miyata type was 0.034880, between Metagonimus Miyata type and M. takahashii was 0.028098, between M. yokogawai and M. takahashii was 0.018179. It is suggested that Metagonimus Miyata type may be separate species and evolutionize at the older time than the other two species.

MeSH Terms

Animal
Cytochrome-c Oxidase/genetics
Evolution, Molecular
Fishes
Heterophyidae/genetics*
Heterophyidae/classification*
Korea
Mitochondria/enzymology
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
RNA, Helminth/genetics
RNA, Ribosomal/genetics
Rats
Rats, Sprague-Dawley
Variation (Genetics)