J Korean Med Sci.  2002 Dec;17(6):778-783. 10.3346/jkms.2002.17.6.778.

Glutamine on the Luminal Microbial Environment After Massive Small Bowel Resection

• Choi SH
• Lee S
• Lee MD

• KMID: 648787
• DOI: http://doi.org/10.3346/jkms.2002.17.6.778

Abstract

To evaluate the oral glutamine (GLN) on the luminal microbes and bacterial translocation (BT) in short bowel, 45 Wistar rats were utilized in three groups; A (control), and B and C (short bowel, 85% of small bowel resected). The group A was fed with elemental diet (EmD), B with EmD+2% glycine, and C with EmD+2% GLN. The groups B and C were isocaloric and isonitrogenous. Wet weight, DNA, protein, and histomorphometry of the mucosa and parallel microbial culture from cecal contents, caval blood, and tissue blocks of the liver, spleen, and mesenteric lymph nodes were performed on the 5th, 10th, and 15th day. Mucosal growth was higher in group C than B. Colony forming units (CFU) from cecal contents increased more in group B than in C. BTs in A, B, and C were 7/15, 8/15, and 2/15, respectively. Total CFUs in blood and tissues were 5.8X10(4)/g, 5.5X10(6)/g, and 1.8X10(4)/g, respectively. As for BT, the most frequent organism was Klebsiella in A (79.3%), but E. coli in B and C (94.2% and 55.6%). GLN seems to suppress luminal microbes, and reduces BT in short bowel due to enforced barrier function and proliferation of the mucosa.

Keyword

Short Bowel Syndrome; Bacterial Translocation; Glutamine; Adaptation; Intestinal Mucosa

MeSH Terms

Animals
Anti-Infective Agents/therapeutic use
Body Weight/drug effects
DNA/metabolism
Glutamine/*therapeutic use
Intestine, Small/drug effects/microbiology/*surgery
Male
Rats
Rats, Wistar
Stem Cells
Time Factors