J Korean Med Sci.  2000 Apr;15(2):159-166. 10.3346/jkms.2000.15.2.159.

DNA ploidy patterns in gastric adenocarcinoma

• Kim JY
• Cho HJ

• KMID: 648117
• DOI: http://doi.org/10.3346/jkms.2000.15.2.159

Abstract

To assess the value of DNA ploidy, flow cytometric analysis was performed on unfixed fresh materials obtained from 86 patients with gastric cancer who underwent stomach resection. We evaluated the DNA content of gastric carcinoma cells from four different sites and compared it with Ki-67 proliferating activity, and other pathologic parameters. The incidence of aneuploid and diploid was similar (48.8% vs. 51.1%). Early gastric carcinoma showed a higher rate of the diploid pattern (75%) compared to that of advanced gastric carcinoma 7.3%). DNA diploidy was noted increasingly in diffuse-type tumors according to uren, in signet ring cell type tumor according to WHO classification and in orly differentiated tumors (p<0.05). Well and moderately differentiated rcinomas revealed the aneuploid pattern more frequently than poorly fferentiated tumors. The aneuploidy was associated with high S phase fraction d high proliferative index. Aneuploidy was noted in the mucosa adjacent to the mor (26%), in the close normal-looking mucosa (7%) and in the remote rmal-looking mucosa (3%). This result suggest the possible role of field ncerization in the development of gastric adenocarcinoma.

Keyword

Ploidies; Ki-67 Antigen; Adenocarcinoma; Stomach Neoplasms

MeSH Terms

Adenocarcinoma/pathology*
Adenocarcinoma/genetics*
Adenocarcinoma/chemistry
Aged
Aneuploidy*
Cell Differentiation
Cell Division
Female
Flow Cytometry
Gastric Mucosa/pathology
Human
Ki-67 Antigen/analysis
Male
Middle Age
Stomach Neoplasms/pathology*
Stomach Neoplasms/genetics*
Stomach Neoplasms/chemistry