J Korean Med Sci.  1999 Jun;14(3):277-285. 10.3346/jkms.1999.14.3.277.

The metabolic effects of estriol in female rat liver

• Yang JM
• Kim SS
• Kim JI
• Ahn BM
• Choi SW
• Kim JK
• Lee CD
• Chung KW
• Sun HS
• Park DH
• Thurman RG

• KMID: 646306
• DOI: http://doi.org/10.3346/jkms.1999.14.3.277

Abstract

The effects of estriol on oxygen uptake, glucose release, lactate and pyruvate production, beta-hydroxybutyrate and acetoacetate production in perfused rat liver as well as, carbon uptake in rat liver and intracellular calcium in isolated Kupffer cells were investigated. Basal oxygen consumption of perfused liver increased significantly in estriol or ethanol-treated rats. But these increased effects were blocked by gadolinium chloride pretreatment. In a metabolic study, pretreatment with estriol resulted in a decrease in glucose production and in glycolysis while an increase in ketogenesis. A more oxidized redox state of the mitochondria was indicated by increased ratios of perfusate [lactate]/[pyruvate] and decreased ratios of perfusate [beta-hydroxybutyrate]/[acetoacetate]. Carbon uptake of Kupffer-cell increased significantly in estriol-treated rats. But these increased uptake were not shown in rats pre-treated by gadolinium chloride blocking phagocytosis. In isolated Kupffer cells from estriol-treated rats, intracellular calcium was more significantly increased after addition of lipopolysaccharide (LPS) than in controls. These findings suggest that the metabolic effects of estriol (two mg per 100 mg body wt) can be summarized to be highly toxic in rat liver, and these findings suggest that oral administration of estrogens may induce hepatic dysfunctions and play a role in the development of liver disease.

Keyword

Estriol; Metabolism; Kupffer cells; Oxygen Consumption; Calcium

MeSH Terms

3-Hydroxybutyric Acid/metabolism
Acetoacetates/metabolism
Animal
Calcium/metabolism
Carbohydrates/metabolism
Carbon/metabolism
Cells, Cultured
Colloids/metabolism
Estriol/pharmacology*
Estriol/metabolism
Ethanol/pharmacology
Female
Gadolinium/pharmacology
Glucose/biosynthesis
Intracellular Fluid/metabolism
Kupffer Cells/metabolism
Kupffer Cells/cytology
Lactates/metabolism
Lipids/metabolism
Liver/metabolism
Liver/drug effects*
Oxygen Consumption
Phagocytosis
Pyruvic Acid/metabolism
Rats
Rats, Sprague-Dawley