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Int J Stem Cells.  2025 Feb;18(1):37-48. 10.15283/ijsc23183.

Applications of Single-Cell Omics Technologies for Induced Pluripotent Stem Cell-Based Cardiovascular Research

Affiliations
  • 1Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA
  • 2K-BioX, Palo Alto, CA, USA
  • 3Department of Biological Sciences, Sookmyung Women’s University, Seoul, Korea
  • 4Department of Biology, Kyung Hee University, Seoul, Korea
  • 5Department of Systems Biotechnology, Konkuk University, Seoul, Korea
  • 6Asthma Research Division, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH, USA
  • 7Department of Anatomy, College of Medicine, Chung-Ang University, Seoul, Korea
  • 8Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford University, Palo Alto, CA, USA

Abstract

Single-cell omics technologies have transformed our investigation of genomic, transcriptomic, and proteomic landscapes at the individual cell level. In particular, the application of single-cell RNA sequencing has unveiled the complex transcriptional variations inherent in cardiac cells, offering valuable perspectives into their dynamics. This review focuses on the integration of single-cell omics with induced pluripotent stem cells (iPSCs) in the context of cardiovascular research, offering a unique avenue to deepen our understanding of cardiac biology. By synthesizing insights from various single-cell technologies, we aim to elucidate the molecular intricacies of heart health and diseases. Beyond current methodologies, we explore the potential of emerging paradigms such as single-cell/spatial omics, delving into their capacity to reveal the spatial organization of cellular components within cardiac tissues. Furthermore, we anticipate their transformative role in shaping the future of cardiovascular research. This review aims to contribute to the advancement of knowledge in the field, offering a comprehensive perspective on the synergistic potential of transcriptomic analyses, iPSC applications, and the evolving frontier of spatial omics.

Keyword

Transcriptome analysis; Induced pluripotent stem cells; Organoids; Cardiovascular diseases

Figure

  • Fig. 1 Overview of the application of human induced pluripotent stem cells (hiPSCs) in single-cell omics technology and cardiovascular research.


Reference

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