Clin Exp Pediatr.  2025 Feb;68(2):141-152. 10.3345/cep.2024.01585.

Differences in immune cells and gene expression in human milk by parity on integrated scRNA sequencing

• Yi DY
• Park HJ
• Shin MS
• Kim H
• Lee SJ
• Kang I

• KMID: 2564619
• DOI: http://doi.org/10.3345/cep.2024.01585

Abstract

Background
Human breast milk (HBM) is an important source of tolerogenic immune mediators that influence the infant immune system. HBM-derived immune components are affected by various factors; however, few studies have examined the relationship between parity and immune cell profiles of HBM. Purpose: This study aimed to clarify the effects of parity on HBM immune cell heterogeneity and gene expression by integrating and analyzing publicly available single-cell RNAsequencing (scRNA-seq) datasets.
Methods
We clarified the effects of parity on HBM immune cell heterogeneity and gene expression by integrating and analyzing publicly available scRNA-seq datasets.
Results
The proportion of innate immune cells was significantly higher in the primiparous versus multiparous group, whereas the proportion of adaptive immune cells was significantly higher in the multiparous group (P=0.021). The 2 immune clusters were reannotated and classified into monocyte, T/B cell, and CD45¯ groups. The proportions of monocytes and T/B cells were higher in the primiparous and multiparous groups, respectively. In a gene set enrichment analysis of monocytes, genes with a direct role in the infant immune system and immune response-related genes were more highly expressed in the primiparous group.
Conclusion
Our results support the parity-dependent differences in gene expression between innate and adaptive immune cells.

Keyword

Human milk; Single-cell RNA-seq; Parity