Nat Prod Sci.  2024 Jun;30(2):143-147. 10.20307/nps.2024.30.2.143.

Effect of Skin Regeneration of Laminariae Thallus Extract in HaCaT Cell and Zebrafish Larvae

• Lee YJ
• Lee IS
• Kim YS
• Ahn SW

• KMID: 2557842
• DOI: http://doi.org/10.20307/nps.2024.30.2.143

Abstract

Many attempts are being made to find safe and therapeutic materials from natural sources. Laminariae Thallus is said to treat swelling of the face in the Dongui-Bogam and has been used in Traditional Korean Medicine to treat boils and chronic gallstones. Recently Laminariae Thallus has been utilized as a cosmetic material for its moisturizing and whitening properties. In this study, water and ethanolic extracts (30%, 50% and 80%) of Laminariae Thallus were prepared. Their regeneration effects were evaluated by cell proliferation and migration in human keratinocytes (HaCaT cells) and tail fin regeneration assay in zebrafish larvae. An 80% ethanol extract (100 μg/mL) of Laminariae Thallus was found to have the strongest ability on the migration of cells. In the tail fin regeneration experiment using zebrafish larvae, 80% ethanol extract (100 and 50 μg/mL) showed a high regeneration effect compared to the control. In conclusion, this study provides a scientific basis for the use of Laminariae Thallus extract for skin regeneration and suggests that it has great potential as a regenerative material for future natural cosmetics.

Keyword

Skin regeneration; Migration assay; Laminariae Thallus Extract; Dongui-Bogam; Traditional Korean Medicine