Clin Exp Emerg Med.
2024 Mar;11(1):43-50. 10.15441/ceem.23.106.
Protective role of kallistatin in oxygen-glucose deprivation and reoxygenation in human umbilical vein endothelial cells
- Affiliations
-
- 1Department of Emergency Medicine, Seoul National University Bundang Hospital, Seongnam, Korea
- 2Department of Emergency Medicine, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, Korea
- 3Disaster Medicine Research Center, Seoul National University Medical Research Center, Seoul, Korea
- 4Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul, Korea
- KMID: 2555261
- DOI: http://doi.org/10.15441/ceem.23.106
Abstract
Objective
Ischemia-reperfusion (IR) injury is implicated in various clinical diseases. Kallistatin attenuates oxidative stress, and its deficiency has been associated with poor neurological outcomes after cardiac arrest. The present study investigated the antioxidant mechanism through which kallistatin prevents IR injury.
Methods
Human umbilical vein endothelial cells (HUVECs) were transfected with small interfering RNA (siRNA) targeting the human kallistatin gene (SERPINA4). Following SERPINA4 knockdown, the level of kallistatin expression was measured. To induce IR injury, HUVECs were exposed to 24 h of oxygen-glucose deprivation and reoxygenation (OGD/R). To evaluate the effect of SERPINA4 knockdown on OGD/R, cell viability and the concentration of kallistatin, endothelial nitric oxide synthase (eNOS) and total NO were measured.
Results
SERPINA4 siRNA transfection suppressed the expression of kallistatin in HUVECs. Exposure to OGD/R reduced cell viability, and this effect was more pronounced in SERPINA4 knockdown cells compared with controls. SERPINA4 knockdown significantly reduced kallistatin concentration regardless of OGD/R, with a more pronounced effect observed without OGD/R. Furthermore, SERPINA4 knockdown significantly decreased eNOS concentrations induced by OGD/R (P<0.01) but did not significantly affect the change in total NO concentration (P=0.728).
Conclusion
The knockdown of SERPINA4 resulted in increased vulnerability of HUVECs to OGD/R and significantly affected the change in eNOS level induced by OGD/R. These findings suggest that the protective effect of kallistatin against IR injury may contribute to its eNOS-promoting effect.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Morphologic and Time Dependent Changes of Nitric Oxide Synthesis Following Anoxia and Reoxygenation of the Endothelial Cells of the Umbilical Vein
- Time Dependent Changes of Nitric Oxide Synthesis Following Anoxia and Reoxygenation of Endothelial Cells of Umbilical Vein
- Epigallocatechin-3-gallate Regulates Inducible Nitric Oxide Synthase Expression in Human Umbilical Vein Endothelial Cells
- Hypericin, a Naphthodianthrone Derivative, Prevents Methylglyoxal-Induced Human Endothelial Cell Dysfunction
- In Vitro Culture of Endothelial Cell and Smooth Muscle Cell for Studying Vascular Diseases
