Tissue Eng Regen Med.  2024 Apr;21(3):421-435. 10.1007/s13770-023-00614-0.

Fabricating Composite Cell Sheets for Wound Healing: Cell Sheets Based on the Communication Between BMSCs and HFSCs Facilitate Full-Thickness Cutaneous Wound Healing

• Li G
• Wang Q
• Liu H
• Yang Z
• Wu Y
• He L
• Deng X

• KMID: 2554993
• DOI: http://doi.org/10.1007/s13770-023-00614-0

Abstract

BACKGROUND
Insufficient angiogenesis and the lack of skin appendages are critical challenges in cutaneous wound healing. Stem cell-fabricated cell sheets have become a promising strategy, but cell sheets constructed by a single cell type are inadequate to provide a comprehensive proregenerative microenvironment for wound tissue.
METHODS
Based on the communication between cells, in this study, bone marrow mesenchymal stem cells (BMSCs) and hair follicle stem cells (HFSCs) were cocultured to fabricate a composite cell sheet (H/M–CS) for the treatment of fullthickness skin wounds in mice.
RESULTS
Experiments confirmed that there is cell–cell communication between BMSCs and HFSCs, which enhances the cell proliferation and migration abilities of both cell types. Cell–cell talk also upregulates the gene expression of proangiogenic-related cytokines in BMSCs and pro-hair follicle-related cytokines in HFSCs, as well as causing changes in the properties of secreted extracellular matrix components.
CONCLUSIONS
Therefore, the composite cell sheet is more conducive for cutaneous wound healing and promoting the regeneration of blood vessels and hair follicles.

Keyword

Bone marrow mesenchymal stem cell; Hair follicle stem cell; Composite cell-sheet; Cutaneous wound healing