Restor Dent Endod.  2021 Nov;46(4):e48. 10.5395/rde.2021.46.e48.

Bone repair in defects filled with AH Plus sealer and different concentrations of MTA: a study in rat tibiae

• Paz JER
• Costa PO
• Souza AAC
• Oliveira IMd
• Falcão LF
• Falcão CAM
• Ferraz M
• Pinto LSS

• KMID: 2548092
• DOI: http://doi.org/10.5395/rde.2021.46.e48

Abstract

Objectives
This study aimed to evaluate the effects on bone repair of different concentrations of mineral trioxide aggregate (MTA) added to AH Plus.
Materials and Methods
Bone tissue reactions were evaluated in 30 rats (Rattus norvegicus) after 7 and 30 days. In the AH + MTA10, AH + MTA20, and AH + MTA30 groups, defects in the tibiae were filled with AH Plus with MTA in proportions of 10%, 20% and 30%, respectively; in the MTA-FILL group, MTA Fillapex was used; and in the control group, no sealer was used. The samples were histologically analyzed to assess bone union and maturation. The Kruskal-Wallis and Mann-Whitney tests were performed for multiple pairwise comparisons (p ≤ 0.05).
Results
At the 7-day time point, AH + MTA10 was superior to MTA-FILL with respect to bone union, and AH + MTA20 was superior to MTA-FILL with respect to bone maturity (p < 0.05). At the 30-day time point, both the AH + MTA10 and AH + MTA20 experimental sealers were superior not only to MTA-FILL, but also to AH + MTA30 with respect to both parameters (p < 0.05). The results of the AH + MTA10 and AH + MTA20 groups were superior to those of the control group for both parameters and experimental time points (p < 0.05).
Conclusions
The results suggest the potential benefit of using a combination of these materials in situations requiring bone repair.

Keyword

AH Plus; Bone regeneration; Mineral trioxide aggregate; MTA Fillapex; Rattus norvegicus; Root canal sealers

Figure

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  Figure 1 Histological images representative of the bone repair observed after filling experimental defects created in rat tibiae with AH + MTA10 (A, B), AH + MTA20 (C, D), and AH+MTA30 (E, F), MTA Fillapex sealer (G, H), and the control group (I, J). At 7 days, only a small bone defect and immature bone consisting of osteoblasts and bone matrix (upward-pointing arrow) can be seen in the AH + MTA10 group, and residual material is also observed (downward-pointing arrow) (A). Differentiation of immature cells into osteoblasts and the deposition of varying amounts of collagen fibers predominate in the other groups (C, D, G, and I). At 30 days, closure of the experimental defect with the formation of a thick cortical bone bridge, morphologically similar to the bone adjacent to the defect, can be seen in the AH + MTA20 group (D). Mineralized, lamellar and irregular compact bone of variable thickness, and persistent medullary spaces (asterisks) can be seen in the other groups (B, F, H, and J) (hematoxylin and eosin stain; original magnification × 200).AH + MTA10, AH Plus sealer and mineral trioxide aggregate in proportions of 10%; AH + MTA20, AH Plus sealer and mineral trioxide aggregate in proportions of 20%; AH + MTA30, AH Plus sealer and mineral trioxide aggregate in proportions of 30%.

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