Nutr Res Pract.  2023 Aug;17(4):660-669. 10.4162/nrp.2023.17.4.660.

Resveratrol promotes mitochondrial energy metabolism in exerciseinduced fatigued rats

• Lou X
• Hu Y
• Ruan R
• Jin Q

• KMID: 2545183
• DOI: http://doi.org/10.4162/nrp.2023.17.4.660

Abstract

BACKGROUND/OBJECTIVES
To investigate the effect and regulatory mechanism of resveratrol supplementation on the mitochondrial energy metabolism of rats with exerciseinduced fatigue.
MATERIALS/METHODS
Forty-eight Sprague-Dawley male rats were divided randomly into a blank control group (C), resveratrol group (R), exercise group (E), and exercise and resveratrol group (ER), with 12 rats in each group. Group ER and group E performed 6-wk swimming training with 5% wt-bearing, 60 min each time, 6 days a wk. Group ER was given resveratrol 50 mg/kg by gavage one hour after exercise; group R was only given resveratrol 50 mg/kg by gavage; group C and group E were fed normally. The same volume of solvent was given by gavage every day.
RESULTS
Resveratrol supplementation could reduce the plasma blood urea nitrogen content, creatine kinase activity, and malondialdehyde content in the skeletal muscle, increase the total superoxide dismutase activity in the skeletal muscle, and improve the fatigue state. Resveratrol supplementation could improve the activities of Ca²⁺ -Mg²⁺ -ATPase, Na⁺ -K⁺ -ATPase, succinate dehydrogenase, and citrate synthase in the skeletal muscle. Furthermore, resveratrol supplementation could up-regulate the sirtuin 1 (SIRT1)-proliferator-activated receptor gamma coactivator-1α (PGC-1α)-nuclear respiratory factor 1 pathway.
CONCLUSIONS
Resveratrol supplementation could promote mitochondrial biosynthesis via the SIRT1/PGC-1α pathway, increase the activity of the mitochondrial energy metabolismrelated enzymes, improve the antioxidant capacity of the body, and promote recovery from exercise-induced fatigue.

Keyword

Fatigue; resveratrol; mitochondrial turnover; energy metabolism

Figure

• 

  Fig. 1 Changes in (A) Ca2+-Mg2+-ATPase and (B) Na+-K+-ATP activity in the skeletal muscle.C, control group; R, resveratrol group; E, exercise group; ER, exercise and resveratrol group.Note: *P < 0.05, **P < 0.01.

• 

  Fig. 2 Changes in the (A) SDH, (B) CS activity, and (C) COX content in the skeletal muscle.C, control group; R, resveratrol group; E, exercise group; ER, exercise and resveratrol group; SDH, succinate dehydrogenase; CS, citrate synthase activity; COX, cytochrome-c oxidase.Note: *P < 0.05.

• 

  Fig. 3 mRNA expression level of (A) SIRT1, (B) PGC-1α, and (C) NRF-1 in the skeletal muscle.C, control group; R, resveratrol group; E, exercise group; ER, exercise and resveratrol group; SIRT1, sirtuin 1; PGC-1α, peroxisome proliferator-activated receptor gamma coactivator-1α; NRF-1, nuclear respiratory factor 1.Note: *P < 0.05, **P < 0.01.

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