Nat Prod Sci.  2023 Jun;29(2):113-119. 10.20307/nps.2023.29.2.113.

Anti-influenza Compounds Isolated from Descurainia sophia Seeds

• Yang WS
• Ma CJ

• KMID: 2544460
• DOI: http://doi.org/10.20307/nps.2023.29.2.113

Abstract

Descurainia sophia seeds methanol extract showed significant anti-influenza activity and we tried to isolate anti-influenza compounds from the D. sophia extract. D. sophia seeds were extracted with 80% methanol and fractionated with n-hexane, ethyl acetate, CHCl ₃ and n-butanol. The anti-influenza activity of each fraction was assessed using sulforhodamine B (SRB) method in A549 cells, human-derived lung cancer cells. The ethyl acetate and CHCl ₃ fractions showed the most potent anti-influenza activity. Seven compounds were isolated from CHCl 3 fraction and identified 1-decanol (1), 2-(3,4-dihydroxy-2-methylenebutoxy)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol (2), daucosterol (3), isorhamnetin (4), quercetin (5), sinapic acid (6), and helveticoside (7) by spectroscopic data such as UV, IR, ¹ H-NMR, ¹ C-NMR and mass spectroscopy. Anti-influenza activities of isolated compounds were evaluated using SRB method in A549 cells. Compounds 3, 4 and 7 had significant antiinfluenza activity in a dose-dependent manner.

Keyword

Descurainia sophia Seeds; anti-influenza; daucosterol; isorhamnetin; helveticoside