Tissue Eng Regen Med.
2023 Apr;20(2):309-322. 10.1007/s13770-022-00517-6.
Chitosan Nerve Grafts Incorporated with SKP-SC-EVs Induce Peripheral Nerve Regeneration
- Affiliations
-
- 1School of Biology and Basic Medical Sciences, Soochow University, Suzhou, China
- 2Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-Innovation Center of Neuroregeneration, NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products, Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair, Nantong University, 19 Qixiu Road, Nantong 226001, Jiangsu, China
- 3Clinical Medical Research Center, The Third Affiliated Hospital of Soochow University, Changzhou, China
- KMID: 2541495
- DOI: http://doi.org/10.1007/s13770-022-00517-6
Abstract
- BACKGROUND
Repair of long-distance peripheral nerve defects remains an important clinical problem. Nerve grafts incorporated with extracellular vesicles (EVs) from various cell types have been developed to bridge peripheral nerve defects. In our previous research, EVs obtained from skin-derived precursor Schwann cells (SKP-SC-EVs) were demonstrated to promote neurite outgrowth in cultured cells and facilitate nerve regeneration in animal studies.
METHODS
To further assess the functions of SKP-SC-EVs in nerve repair, we incorporated SKP-SC-EVs and Matrigel into chitosan nerve conduits (EV-NG) for repairing a 15-mm long-distance sciatic nerve defect in a rat model. Behavioral analysis, electrophysiological recording, histological investigation, molecular analysis, and morphometric assessment were carried out.
RESULTS
The results revealed EV-NG significantly improved motor and sensory function recovery compared with nerve conduits (NG) without EVs incorporation. The outgrowth and myelination of regenerated axons were improved, while the atrophy of target muscles induced by denervation was alleviated after EVs addition.
CONCLUSION
Our data indicated SKP-SC-EVs incorporation into nerve grafts represents a promising method for extended peripheral nerve damage repair.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- A Histological Study of Peripheral Nerve Regeneration in Rats
- Comparison of the Nerve Regenerating Effect of Inside-out Vein Grafts, Muscle-filled Inside-out Vein Grafts, and Fat-interposed Inside-out Vein Grafts in Rats
- The effect of several nerve conduit on peripheral nerve regeneration in rabbits
- Treatment of the Traumatic Neuroma
- Effect of Basic Fibroblast Growth Factor on the Regeneration of the Allografted Sciatic Nerve in Rat
