J Korean Acad Pediatr Dent.  2022 Aug;49(3):300-309. 10.5933/JKAPD.2022.49.3.300.

Effects of Ginsenoside Rb1 Loaded Films on Oral Wound Healing

• Lee JH
• Park SH
• Mohammed AN
• Lee MH
• Kweon DK
• Chae Y
• Lee K
• Kim M
• Lee H
• Choi S
• Nam OH

• KMID: 2533713
• DOI: http://doi.org/10.5933/JKAPD.2022.49.3.300

Abstract

This study aimed to evaluate the effects of two ginsenoside Rb1 (G-Rb1) loaded films on oral wound healing. Two types of G-Rb1 films, G-Rb1 loaded carboxymethyl cellulose (GCMC) film and G-Rb1 loaded hyaluronic acid (GHA) film, were developed. A total of 36 Sprague-Dawley rats were divided into 3 groups: control, GCMC, and GHA. After wound formation on midpalate, the control group was left without treatment, whereas the experimental groups had films attached. The specimen was analyzed clinically and histologically after 7 and 21 days. For clinical analysis, the area of incompletely re-epithelialized wound was measured. For histological analysis, the distance between the margins of the wound (soft tissue gap) was measured and the percentage of the collagen-stained area on the specimen was calculated. In clinical and soft tissue gap analysis, the GCMC group presented improved healing compared to the GHA group and the control at day 7 (p < 0.05). And, both GCMC (9.74 ± 10.12%) and GHA groups (19.50 ± 14.47%) presented greater collagen-positive pixels compared to control (0.89 ± 1.60%) at day 7 (p < 0.05). However, there were no differences in these parameters among the groups on day 21. Therefore, G-Rb1 loaded films improved oral wound healing.

Keyword

Ginsenoside Rb1; Carboxymethyl cellulose; Hyaluronic acid; Wound healing; Reepithelialization