Tuberc Respir Dis.  1973 Mar;20(1):20-29. 10.4046/trd.1973.20.1.20.

Epidemiological and Bacteriological Studies of Bovine Tuberculosis in Korea

Abstract

( Report III) A) Mycobacteria Isolated from Ranch Soils. A study was carried out on the isolations of mycobacteria from 33 samples which were collected from 13 ranches in Kyong-gi Province.
Results
obtained were as follows. (1) Twenty-six isolates from 33 soil samples were classified according to Runyon's Scherna; 10 strains or 38.5% were Group II scotochromogens. seven or 26% were Group III nonchromogens and nine 01: 34.6% were Group IV rapid growers. (2) The ten strains of Runyon Group II were subdivided into six strains of M. scrofulaceum and four strains of M. gordonae which were 23. 1 % and 15. 4% respectively of the 26 total strains isolated . The seven strains of Runyon Group III were subdivided into three strains of M. terrae complex. One strain of M. gastri and three unidentified which were 11.5%. 3.9% and 11.5% respectively of the 26 total strains isolated. B) Cross-Reaction between Specifidty of Tuberculins Prepared with Various Mycodacteria Isolated from Tuberculin Reactive Dairy Cattle. Guinea pigs were sensitized by the strains isolated from tuberculin reactive dairy cattle and were observed for cross reactions using the following tuberculins: (1) Heat Concentrated Synthetic Mediurn tuberculin for human and bovine types. (2) Tuberculin prepared by a modification of the Old Tuberculin Method for M. terrae complex, M. intracellula re ( BatteYJ , M. gordonae, and M. fortuitum . ( 3) U. S. Standard Tuberculins, O. T. , for the same strains as listed under number 2. Judging from the results of skin tests, the degree of heterologous reactions was as high as that for the homologous reactins. Therefore it is questionable whether or not accurate discriminations can be made between bovine tuberculosis a nd other mycobacterial infections, using conventional tuberculin.

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