Tuberc Respir Dis.  1974 Jun;21(2):79-84. 10.4046/trd.1974.21.2.79.

Studies on the Lymphocyte Culture in Patients with Antituberculous Drug Hypersensitivity

• KMID: 2529020
• DOI: http://doi.org/10.4046/trd.1974.21.2.79

Abstract

Lymphoblastogenesis (transformation of lymphocyte) from peripheral blood into blast-like cell in vitro patients with certain drug reactions in the presence of the appropriate drug has been used as a test for drug hypersensitivity since Ripps, Fellner and Hirschhorn in 1965. In this paper, eleven patients with active pulmonary tuberculosis who were receiving a trial-chemotherapy with INH, PAS and SM were investigated, and in only one of these the culture was positive. In this case (10th patient of this series, case 10) the result of the lymphocyte culture with PAS showed. evidence of blastogenesis in about 15 percent in concentration 0.1 5 micro gm and 0.015 micro gm perml. In the control cultures only 1-2% of the lymphocytes were transformed. On the other hand , in the same case (case 10) the result of 3H-thymidine uptake to INH revealed 1273 cpm count in a concentration of 0.06 micro gm per ml. comparing with a control cpm count 269. The degree of lymphocyte culture is know n to be dependent on the dose of the drug ; this in turn means that optimtim concentration for each drug will have to be established .