Tuberc Respir Dis.  1986 Jun;33(2):103-109. 10.4046/trd.1986.33.2.103.

Serum Mediator of Human ARDS

• KMID: 2528704
• DOI: http://doi.org/10.4046/trd.1986.33.2.103

Abstract

The role of neutrophil is important in the pathogenesis of Adult Respiratory Distress Syndrome(ARDS), but there is controversy about the mechanism of neutrophil activation. To evaluate the role of serum mediator in patients of ARDS, we measured adherence and superoxide formation by neutrophil derived from normal healthy control and incuvated with serum of normal control, serum of patient of ARDS, and zymosan activated serum. 1) Neutrophil adherence in patients of ARDS was significantly higher than that of normal (57.17±13.51% vs 22.86±8.12%, P<0.005). 2) Neutrophil adherence in non-recovered patients of ARDS was significantly higher than that of recovered patients of ARDS (60.57±13.61% vs 46.96±6.71%, P<0.01), and there was no difference between septic patients of ARDS and nonseptic patients ARDS. 3) Neutrophil adherence by zymosan activated serum was significantly higher than that of normal control (50.54±11. 93% vs 22.36±8.11%, P<0.011). 4) Neutrophil-superoxide generation by serum of patients of ARDS ￦ as significantly higher than that of normal control (71. 78±74. 5u vs 26.64±26. 27u, P<0.05). 5) Neutrophil-superoxide generation by zymosan activated serum was not significantly higher than that by zymosan free serum. In conclusion, serum of patients with ARDS has hurmoral mediators that inhance and superoxide generation of neutrophil, and the former was dependent on serum complement but the latter was not entirely dependent on serum complement.