J Obes Metab Syndr.  2022 Mar;31(1):81-85. 10.7570/jomes22013.

Mouse Model of Small for Gestational Age Offspring with Catch-up Growth Failure and Dysregulated Glucose Metabolism in Adulthood

• Moon HS
• Kim H
• Kim B
• Kim MS
• Kim JH
• Kwon O

• KMID: 2527736
• DOI: http://doi.org/10.7570/jomes22013

Abstract

Background
We aimed to build mouse models of small for gestational age (SGA), recapitulating failure of catch-up growth and dysregulated metabolic outcomes in adulthood.
Methods
Pregnant C57BL/6 mice were given a protein-restricted diet (PRD; 6% kcal from protein) during pregnancy without (model 1) or with cross-fostering (model 2). Model 3 extended the PRD to the end of the lactation period. Model 4 changed to a 9% PRD without cross-fostering.
Results
Model 1 yielded a reduced size of offspring with a poor survival rate. Model 2 improved survival but offspring showed early catch-up growth. Model 3 maintained a reduced size of offspring after weaning with a higher body mass index and blood glucose levels in adult stages. Model 4 increased the survival of the offspring while maintaining a reduced size and dysregulated glucose metabolism.
Conclusion
Models 3 and 4 are suitable for studying SGA accompanying adulthood short stature and metabolic disorders.

Keyword

Small for gestational age; Growth failure; Glucose metabolism disorders; Obesity