J Korean Diabetes.  2021 Mar;22(1):60-70. 10.4093/jkd.2021.22.1.60.

Effects of Jerusalem Artichoke Extract and Inulin on Blood Glucose Levels and Insulin Secretion in Streptozotocin Induced Diabetic Mice

• Kim SH
• Kim BK
• Park BY
• Kim JM
• Lee YJ
• Lee MK
• Yee ST
• Kang MY

• KMID: 2526182
• DOI: http://doi.org/10.4093/jkd.2021.22.1.60

Abstract

Background
To determine the effects of Jerusalem Artichoke extract (JAE) and inulin on blood glucose levels and insulin secretion in streptozotocin (STZ)-induced diabetic mice.

Methods
Thirty four mice were divided into a normal control group and three experimental groups: diabetic control, JAE, and inulin. STZ (50 mg/kg) was injected intraperitoneally to induce diabetes in the three experimental groups. The JAE and inulin groups were fed 10 g/kg JAE or fed 1 g/kg inulin, respectively, for 6 weeks. Fasting glucose was checked weekly. After 6 weeks, the oral glucose tolerance test (OGTT) was performed, and the insulin level was checked.

Results
Four mice from the JAE group (n = 9) died and autopsies revealed inflammation and ulceration of skin lesions on the chest areas. Fasting glucose levels were not decreased in the inulin or JAE group relative to diabetic control group. In the OGTT at 60 minutes and 120 minutes, the serum glucose levels were significantly higher in the inulin group (572.6 ± 52.0 mg/dL and 555.8 ± 72.9 mg/dL, respectively) than in diabetic control group (484.3 ± 81.6 mg/dL and 467.3 ± 111.1 mg/dL, respectively). Insulin levels were not increased in the inulin group relative to the diabetic control group.

Conclusion
These results indicate that JAE and inulin might not be useful therapeutic strategies for diabetes mellitus and indiscreet intake of Jerusalem Artichoke could exacerbate to diabetes.

Keyword

Diabetes mellitus; Glucose; Helianthus; Insulin; Inulin

Figure

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  Fig. 1. The blood glucose levels in normal, diabetes, Jerusalem Artichoke, and inulin group. a,b,c Means not sharing a common letter are significantly different among groups (P < 0.05).

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  Fig. 2. The result of oral glucose tolerance test (OGTT) in normal, diabetes, and inulin group. a,b,c Means not sharing a common letter are significantly different among groups (P < 0.05).

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  Fig. 3. Insulin (A) and GLP-1 (B) levels in normal, diabetes, and inulin group. GLP-1, glucagon-like peptide-1. a Means not sharing a common letter are significantly different among groups (P < 0.05).

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  Fig. 4. Normal group mouse (A) vs Jerusalem Artichoke group mouse with complications (B). (A) Normal group mice showed normal skin, subcutaneous tissue and muscle. (B) Jerusalem Artichoke group mice macroscopically showed swollen skin and microscopically showed skin ulcer and inflammatory cells infiltration into subcutaneous tissue and skeletal muscle. H&E stain, ×40 (A, B).

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