Maxillofac Plast Reconstr Surg.  2021;43(1):28. 10.1186/s40902-021-00314-6.

The effect of 4-hexylresocinol administration on SCC-9 cells: mass spectrometric identification of proteins and cDNA microarray analysis

• Kang YJ
• Kim SG

• KMID: 2525712
• DOI: http://doi.org/10.1186/s40902-021-00314-6

Abstract

Background
In stress situations, bacteria produce dormancy-inducing factors to stop cell growth. The dormancyinducing factors may have an inhibitory effect on tumor cell growth. Here we analyzed the differentially expressed protein profiles after 4-hexylresorcinol (4HR), one of the dormancy-inducing factors, administration using in vitro oral squamous carcinoma cells (SCC-9). Method: The control group was SCC-9 cells culture without 4HR administration. The experimental group received 10 μg/mL of 4HR. Collected proteins from each group were loaded for 2D electrophoresis. Among the separated proteins, 20 differentially expressed proteins were selected and processed for LC-MS/MS.
Results
In proteomic analysis, the expression of keratin 1, keratin 10, and histone H2B were increased. In cDNA microarray assay, the genes related to the cellular differentiation (involucrin, keratin 13, 14) were highly expressed in the 4HR treated group (fold ratio > 2.0; Table 2). Interestingly, histone family was upregulated in the cDNA microarray assay.
Conclusion
The administration of 4HR on SCC-9 cells increased epithelial cell differentiation markers and histone.

Keyword

4-hexylresorcinol; Oral squamous cell carcinoma; Keratin; Histone