J Vet Sci.  2021 Nov;22(6):e66. 10.4142/jvs.2021.22.e66.

Next-generation sequencing for the genetic characterization of Maedi/Visna virus isolated from the northwest of China

• Zhao L
• Zhang L
• Shi X
• Duan X
• Li H
• Liu S

• KMID: 2522881
• DOI: http://doi.org/10.4142/jvs.2021.22.e66

Abstract

Background
Maedi/Visna virus (MVV) is a contagious viral pathogen that causes considerable economic losses to the sheep industry worldwide.
Objectives
In China, MVV has been detected in several regions, but its molecular characteristics and genetic variations were not thoroughly investigated.
Methods
Therefore, in this study, we conducted next-generation sequencing on an MVV strain obtained from northwest China to reveal its genetic evolution via phylogenetic analysis.
Results
A MVV strain obtained from Inner Mongolia (NM) of China was identified. Sequence analysis indicated that its whole-genome length is 9193 bp. Homology comparison of nucleotides between the NM strain and reference strains showed that the sequence homology of gag and env were 77.1%–86.8% and 67.7%–75.5%, respectively. Phylogenetic analysis revealed that the NM strain was closely related to the reference strains isolated from America, which belong to the A2 type. Notably, there were 5 amino acid insertions in variable region 4 and a highly variable motif at the C-terminal of the surface glycoprotein (SU5).
Conclusions
The present study is the first to show the whole-genome sequence of an MVV obtained from China. The detailed analyses provide essential information for understanding the genetic characteristics of MVV, and the results enrich the MVV library.

Keyword

Maedi/Visna virus; next-generation sequencing; phylogenetic analysis; sheep lung