J Vet Sci.  2021 Sep;22(5):e69. 10.4142/jvs.2021.22.e69.

Detection and molecular characteristics of bovine rotavirus A in dairy calves in China

• Liu X
• Yan N
• Yue H
• Wang Y
• Zhang B
• Tang C

• KMID: 2520766
• DOI: http://doi.org/10.4142/jvs.2021.22.e69

Abstract

Background
Bovine group A rotavirus (BoRVA) is a major cause of severe gastroenteritis in newborn dairy calves. Only one study has investigated the G and P genotypes among dairy calves in a few regions of China, which were G6 and P[5]. Therefore, data on the prevalence and molecular characteristics of BoRVA in dairy calves in China remains limited.
Objectives
The purpose of this study was to investigate the prevalence and molecular characteristics of BoRVA in dairy calves in China.
Methods
269 dairy calves diarrheic samples from 23 farms in six provinces in China were collected to detect BoRVA using reverse transcription polymerase chain reaction.
Results
71% of samples were determined to be BoRVA-positive. Two G genotypes (G6, G10) and two P genotypes (P[1], P[5]) were identified, and G6P[1] BoRVA was the predominant strain. Moreover, the VP7 and VP4 gene sequences of these dairy calf BoRVA strains revealed abundant genetic diversity. Interestingly, eight out of 17 complete G6 VP7 sequences were clustered into G6 lineage VI and analysis showed the strains were closely related to Chinese yak BoRVA strains.
Conclusions
The results of this study show that BoRVA circulates widely among dairy calves in China, and the dominant genotype in circulation is G6P[1], first report on molecular characteristics of complete P[5] VP4 genes in chinese dairy calves. These results will help us to further understand the prevalence and genetic evolution of BoRVA among dairy calves in China and, thus, prevent the disease more effectively.

Keyword

Dairy calf; BoRVA; prevalence; molecular characteristics