Pediatr Gastroenterol Hepatol Nutr.
2021 Sep;24(5):443-454. 10.5223/pghn.2021.24.5.443.
Investigation of Thiol/Disulfide Balance in Obese Rats with Non-Alcoholic Fatty Liver Disease
- Affiliations
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- 1Department of Pediatrics, Faculty of Medicine, Kırıkkale University, Kırıkkale, Turkey
- 2Department of Pediatric Gastroenterology, Faculty of Medicine, Kırıkkale University, Kırıkkale, Turkey
- 3Department of Internal Medicine, Faculty of Veterinary Medicine, Kırıkkale University, Kırıkkale, Turkey
- 4Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Kırıkkale University, Kırıkkale, Turkey
- 5Department of Biochemistry, Faculty of Medicine, Yıldırım Beyazıt University, Ankara, Turkey
- KMID: 2520139
- DOI: http://doi.org/10.5223/pghn.2021.24.5.443
Abstract
- Purpose
Due to the increasing prevalence of obesity worldwide, non-alcoholic fatty liver disease (NAFLD) has reached epidemic dimensions over time. NAFLD is the most common cause of childhood chronic liver disease. There is a relationship between NAFLD and oxidative stress. This study aims to investigate the changes in thiol/disulfide homeostasis parameters to determine the oxidant/antioxidant balance in obese rats with diet-induced NAFLD and healthy rats.
Methods
Twelve Wistar albino rats were used in this study. Experimentally produced NAFLD obese rats (n=6) and healthy rats were compared. Experimental NAFLD model was created with a special fatty liver diet (Altromin ® C1063, Fatty Liver Diet, Exclusivet, Lage, Germany). The biochemical and histopathological features of the groups, as well as serum thiol/disulfide homeostasis parameters, were analyzed and compared.
Results
In the experimentally induced NAFLD rat model, they gained more weight than the control group. Steatosis (at least grade 2) occurred in all rats fed with special fatty liver diet for 12 weeks. Histopathologically, no high-grade inflammation was observed in rats with experimental NAFLD after feeding a diet for 12 weeks. Results revealed that aspartate transaminase and alanine transaminase levels were high, albumin levels were low, oxidant stress parameters increased, and antioxidant thiol groups decreased.
Conclusion
Experimental NAFLD is characterized by increased oxidant stress accompanying fatty tissue in the liver. Analysis of thiol/disulfide homeostasis parameters in NAFLD can be used in further studies to develop effective treatment options.
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