J Korean Ophthalmol Soc.  2021 Jul;62(7):976-982. 10.3341/jkos.2021.62.7.976.

Effect of Anti-vascular Endothelial Growth Factor on the Matrix Metalloproteinase Activity in Trabecular Meshwork Cells

• Park HJ
• Shin JW
• Kim JW

• KMID: 2518501
• DOI: http://doi.org/10.3341/jkos.2021.62.7.976

Abstract

Purpose
To investigate the effects of anti-vascular endothelial growth factor (anti-VEGF) on the activity of matrix metalloproteinase (MMP) in human trabecular meshwork cells (HTMC).
Methods
Primary HTMC cultures were exposed to 0, 0.25, and 0.50 mg/mL anti-VEGF bevacizumab (BV) for 24 hours. The permeability through the trabecular meshwork cell monolayer was assessed using carboxyfluorescein and trans-epithelial endothelial electrical resistance (TEER). The levels of MMP-1/-2 were measured by Western blotting and the production of nitric oxide (NO) was assessed with the Griess assay.
Results
Bevacizumab at 0.50 mg/mL decreased the permeability of carboxyfluorescein significantly (p = 0.017) and did not affect TEER (p = 0.308). Administration of 0.50 mg/mL BV decreased MMP-1 and MMP-2 activities (p = 0.014, p = 0.016, respectively) and inhibited NO production significantly (p = 0.023).
Conclusions
Anti-VEGF decreased the permeability through the HTMC monolayer, which was accompanied by decreased MMP-1/-2 activity and limited NO production.

Keyword

Anti-vascular endothelial growth factor, Bevacizumab, Matrix metalloproteinase, Nitric oxide, Trabecular meshwork