J Korean Dysphagia Soc.  2021 Jan;11(1):9-14. 10.34160/jkds.2021.11.1.002.

Novel Animal Model of Oral Dysphagia Following Hemiglossectomy

• Kim MJ
• Tri HM
• Kim JM
• Choi ME
• Kim YM
• Choi JS
• Kim JW

• KMID: 2512563
• DOI: http://doi.org/10.34160/jkds.2021.11.1.002

Abstract

Objective
This study aimed to establish an animal model of oral dysphagia for translational research purposes.
Methods
Four week old C57BL/6 mice were randomized to a surgical partial glossectomy group (horizontal resection, n=13 vs. vertical resection, n=15) and a nonsurgical group (control, n=7). The survival rate, mouse weight, amount of food, and water consumption were compared. Comparisons were made using a Kruskall Wallis test, and survival was analyzed using Kaplan-Meier method.
Results
Eleven of the 13 (84.6%) horizontal resection animals and 11 of the 15 (73.3%) vertical resection animals survived to the study endpoint. Compared to the non-surgical control mice, the mice that had undergone a horizontal and vertical resection had a significantly lower mean body weight (P＜0.05) during experimental day (ED) 5, with the greatest dysphagia observed in ED 2. The horizontal resection-type mice showed greater mean body weight loss and food consumption in the early stages of the experiment than the vertical resection-type mice. In contrast, the horizontal resection-type mice showed greater body weight gain and food consumption in the late stages of the experiment.
Conclusion
This study provides novel evidence that the proper timing for an animal model of oral dysphagia was within ED 5; severe dysphagia was observed in ED 2. The horizontal-type partial glossectomy had a better survival rate than the vertical-type model. Furthermore, the prolonged dysphagia model mimics the vertical resection-type mice.

Keyword

Animal model; Dysphagia; Oral