Pediatr Gastroenterol Hepatol Nutr.
2021 Jan;24(1):30-37. 10.5223/pghn.2021.24.1.30.
Evidence for Differentiation of Colon Tissue Microbiota in Patients with and without Postoperative Hirschsprung's Associated Enterocolitis: A Pilot Study
- Affiliations
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- 1Division of Pediatric Gastroenterology, Department of Pediatrics, Medical University of South Carolina, Charleston, SC, USA
- 2South Carolina Clinical and Translational Research Institute, Charleston, SC, USA
- 3Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, SC, USA
- 4Program for Human Microbiome Research, Biomedical Informatics Center, Departments of Public Health Sciences in College of Medicine; Oral Health Sciences in College of Dental Medicine; and Healthcare Leadership and Management in College of Health Professions, Medical University of South Carolina, Charleston, SC, USA
- KMID: 2510622
- DOI: http://doi.org/10.5223/pghn.2021.24.1.30
Abstract
- Purpose
To investigate the differences in the colon microbiota composition of Hirschsprung's disease (HSCR) patients with and without a history of postoperative Hirschsprung's associated enterocolitis (HAEC).
Methods
Colon tissue microbiota was characterized by bacterial deoxyribonucleic acid (DNA) extraction and 16S rDNA sequencing for taxonomic classification and comparison.
Results
The sequence diversity richness within samples was significantly higher in samples from patients with a history of postoperative HAEC. We observed an increased relative abundance of the phyla Bacteroidetes, Firmicutes and Cyanobacteria in HAEC patients and Fusobacteria, Actinobacteria and Proteobacteria in HSCR patients and, an increased relative abundance of the genera Dolosigranulum, Roseouria and Streptococcus in HAEC patients and Propionibacterium and Delftia in HSCR patients.
Conclusion
Our findings provide evidence that the colon tissue microbiota composition is different in HSCR patients with and without postoperative HAEC.
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