J Korean Foot Ankle Soc.  2020 Dec;24(4):142-147. 10.14193/jkfas.2020.24.4.142.

Relationship between the Diurnal Temperature Range and Wound Healing of Diabetic Foot: Animal Study

• Won SH
• Chun DI
• Cho J
• Park IK
• Yi Y

• KMID: 2509535
• DOI: http://doi.org/10.14193/jkfas.2020.24.4.142

Abstract

Relationship between the Diurnal Temperature Range and Wound Healing of Diabetic Foot: Animal StudyPurpose: Diabetic foot ulcers are closely related to body surface heat, which can be affected easily by temperature differences. This study examined the correlation between the healing process of diabetic wounds and abnormal diurnal temperature through an animal study.
Materials and Methods
Rats in the abnormal diurnal temperature group and control group were given a 10 mm sized full-thickness skin ulcer. Wound size progression was observed in both groups. H&E and Masson’s trichrome staining was performed at 14 days after wound formation, and the number of vessels per unit area and histology analysis were performed. The changes in the ulcer were measured through three dimensional cross-section area using INSIGHT^® devices.
Results
The wound recovery period (granulation ingrowing) was 24 days in the abnormal diurnal temperature model and 20 days in the control group. The thickness of scar tissue was 402±23.19 μm in the control group and 424.5±36.94 μm in the diurnal temperature model. Neovascular formation was counted as 5.1±0.97 for the control group and 4.16±0.94 for the diurnal temperature model group.
Conclusion
Delayed and inferior diabetic wound healing was observed in the abnormal diurnal temperature group, which was characterized by greater diurnal variations than the typical growth environment.

Keyword

Diabetic foot; Amputation; Animal study; Temperature; Wound healing

Figure

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  Fig. 1 Schematic diagram describing wound formation in diabetic rat. Two full thickness skin ulcers with same size (A) were made on the bilateral infrascapular regions (B).

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  Fig. 2 Comparison of wound recovery in diurnal temperature group and control group. (A) Significantly delayed wound recovery was detected in the diurnal temperature group since three days after the wound formation. (B) Extent of wound recovery was evaluated by estimating the circumferential area of the wound.

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  Fig. 3 Histologic analysis of the scar tissue for control group (A) and diurnal temperature group (B). Collagen fibers were stained with Masson’s trichrome staining (left; magnification, ×100) and wound cross section was stained with hematoxylin and eosin (H&E) staining (right; magnification, ×100). The control group showed higher degree of fibrosis and collagen fiber formation compared with the diurnal temperature group.

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  Fig. 4 (A) Neovascular formation of the wound tissue. Greater amount of neovascular formation (arrow) was found in control group (C) compared with diurnal temperature group (B) with statistically significance (H&E stain, ×100).

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