J Vet Sci.  2020 Mar;21(2):e31. 10.4142/jvs.2020.21.e31.

etection of infectious hypodermal and hematopoietic necrosis virus and white spot syndrome virus in whiteleg shrimp (Penaeus vannamei) imported from Vietnam to South Korea

• Park SC
• Choi SK
• Han SH
• Park S
• Jeon HJ
• Lee SC
• Kim KY
• Lee YS
• Kim JH
• Han JE

• KMID: 2506190
• DOI: http://doi.org/10.4142/jvs.2020.21.e31

Abstract

n this study, whiteleg shrimp (Penaeus vannamei) imported from Vietnam were collected from South Korean markets, and examined for 2 viruses: infectious hypodermal and hematopoietic necrosis virus (IHHNV, recently classified as decapod penstyldensovirus-1), and white spot syndrome virus (WSSV). Among 58 samples, we detected IHHNV in 23 samples and WSSV in 2 samples, using polymerase chain reaction and sequencing analyses. This is the first report of IHHNV and WSSV detection in imported shrimp, suggesting that greater awareness and stricter quarantine policies regarding viruses infecting shrimp imported to South Korea are required.

Keyword

Infectious hypodermal and hematopoietic necrosis virus; white spot syndrome virus; South Korea; quarantine; Vietnam

Figure

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  Fig. 1. Phylogenetic analyses of the representative IHHNV (A) and WSSV (B) detected in this study. Maximum-likelihood trees were reconstructed based on the obtained sequences of the IHHNV-positive sample (sample No. 19-005-h5 corresponding to the strain IHHNV-KK-VIET1, MN481525 [390 bp]) and WSSV-positive sample (sample No. 19-005-h5 corresponding to the strain WSSV-KK-VIET1, MN481520 [936 bp]), respectively. Tiger shrimp, Penaeus monodon (AY124937.1), and Jamaican Bromeliad Crab (Metopaulias depressus) WSSV-like virus (KR820241.1) were used as outgroup for IHHNV and WSSV phylogeny, respectively. Numbers at the branches indicate bootstrap values obtained with 1,000 replicates. The scale bar represents 0.005 and 0.1 nucleotide substitutions per site, respectively. IHHNV, infectious hypodermal and hematopoietic necrosis virus; WSSV, white spot syndrome virus.

Reference

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