Ann Clin Microbiol.  2020 Mar;23(1):21-31. 10.5145/ACM.2020.23.1.21.

Frequency of Mycobacterium tuberculosis Among M. tuberculosis Complex Strains Isolated from Clinical Specimen

Affiliations
  • 1Department of Laboratory Medicine, Wonju Severance Christian Hospital, Yonsei University Wonju College of Medicine, Wonju, Korea. u931018@yonsei.ac.kr
  • 2Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Korea.

Abstract

BACKGROUND
Rapid and accurate detection of Mycobacterium tuberculosis (MTB) is of primary importance for infection control and selection of anti-tuberculosis drugs. However, most clinical laboratories report MTB complex (MTC) without reporting MTB because MTC comprising MTB, Mycobacterium bovis, Mycobacterium africanum, Mycobacterium microti, Mycobacterium caprae and Mycobacterium pinnipedii have 99.9% similarity at the nucleotide level and identical 16S rRNA sequences. This study was conducted to analyze the species frequency of MTC isolates obtained from clinical specimen.
METHODS
Of 310 MTC isolates obtained from clinical samples in a tertiary care hospital from February 2017 to August 2018, MolecuTech Real TB-Taq (YD Diagnostics, Korea) real-time PCR was performed, specifically to detect MTB. For DNA showing MTB negative results by MTB-specific real-time PCR or pyrazinamide-resistant strains, PCR-based MTC typing, spoligotyping, and exact tandem repeat D gene sequencing were performed.
RESULTS
All the 310 MTC isolates were identified to be MTB. Two MTB strains of East-African-Indian 4-Vietnam genotype, which have not been reported in Korea, were also found.
CONCLUSION
There was no zoonotic tuberculosis in this study. Since we investigated only 310 MTC isolates detected in only one medical institution, multi-center study is needed to accurately know the prevalence of zoonotic tuberculosis in Korea.

Keyword

Mycobacterium tuberculosis complex; Sequence analysis; Spoligotyping

MeSH Terms

DNA
Genotype
Goats
Infection Control
Korea
Mycobacterium bovis
Mycobacterium tuberculosis*
Mycobacterium*
Prevalence
Real-Time Polymerase Chain Reaction
Sequence Analysis
Tandem Repeat Sequences
Tertiary Healthcare
Tuberculosis*
DNA

Reference

References

1. Rodriguez-Campos S, Smith NH, Boniotti MB, Aranaz A. Overview and phylogeny of Mycobacterium tuberculosis complex organisms: Implications for diagnostics and legislation of bovine tuberculosis. Res Vet Sci. 2014; 97:S5–19.
Article
2. Rastogi N, Legrand E, Sola C. The mycobacteria: An introduction to nomenclature and pathogenesis. Rev Sci Tech. 2001; 20:21–54.
Article
3. Hwang S, Oh KJ, Jang IH, Uh Y, Yoon KJ, Kim HY, et al. Evaluation of the diagnostic performance of the AdvanSure TB/NTM realtime PCR kit for detection of mycobacteria. Korean J Clin Microbiol. 2011; 14:55–9.
Article
4. Huard RC, Lazzarini LC, Butler WR, van Soolingen D, Ho JL. PCR-based method to differentiate the subspecies of the Mycobacterium tuberculosis complex on the basis of genomic deletions. J Clin Microbiol. 2003; 41:1637–50.
5. Djelouadji Z, Raoult D, Daffé M, Drancourt M. A single-step sequencing method for the identification of Mycobacterium tuberculosis complex species. PLoS Negl Trop Dis. 2008; 2:e253.
Article
6. Coitinho C, Greif G, Robello C, van Ingen J, Rivas C. Identification of Mycobacterium tuberculosis complex by polymerase chain reaction of exact tandem repeat-D fragment from mycobacterial cultures. Int J Mycobacteriol. 2012; 1:146–8.
Article
7. World Health Organization. Global tuberculosis report 2018. Geneva: WHO press;2018.
8. World Health Organization, Food and Agriculture Organization of the United Nations (FAO) and World Organisation for Animal Health(OIE). Roadmap for zoonotic tuberculosis. Geneva: WHO press;2017.
9. Müller B, Dürr S, Alonso S, Hattendorf J, Laisse CJ, Parsons SD, et al. Zoonotic Mycobacterium bovis-induced tuberculosis in humans. Emerg Infect Dis. 2013; 19:899–908.
10. Dürr S, Müller B, Alonso S, Hattendorf J, Laisse CJ, van Helden PD, et al. Differences in primary sites of infection between zoonotic and human tuberculosis: results from a worldwide systematic review. PLoS Negl Trop Dis. 2013; 7:e2399.
Article
11. Lim SK, Park JY, Park SD, Chang HK. Localized empyema due to Mycobacterium bovis. Korean J Med. 2012; 81:792–6.
12. Brown T, Nikolayevskyy V, Velji P, Drobniewski F. Associations between Mycobacterium tuberculosis strains and phenotypes. Emerg Infect Dis. 2010; 16:272–80.
13. Das S, Das SC, Verma R. Occurrence of RD9 region and 500 bp fragment among clinical isolates of Mycobacterium tuberculosis and Mycobacterium bovis. Microbiol Immunol. 2007; 51:231–4.
14. Kamerbeek J, Schouls L, Kolk A, van Agterveld M, van Soolingen D, Kuijper S, et al. Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology. J Clin Microbiol. 1997; 35:907–14.
Article
15. Azadi D, Motallebirad T, Ghaffari K, Shojaei H. Mycobacteriosis and tuberculosis: laboratory diagnosis. Open Microbiol J. 2018; 12:41–58.
Article
16. Nguyen VA, Choisy M, Nguyen DH, Tran TH, Pham KL, Dinh PT, et al. High prevalence of Beijing and EAI4-VNM genotypes among M. tuberculosis isolates in northern Vietnam: sampling effect, rural and urban disparities. PLoS One. 2012; 7:e45553.
Article
Full Text Links
  • ACM
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2023 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr