J Korean Orthop Assoc.  1974 Jun;9(2):165-172. 10.4055/jkoa.1974.9.2.165.

Comparative Study on Phagocytic Activities of Rabbit Macrophages on Staph. Aureus and B. Subtilis in Single and Mixed Suspension

• Kim KW

• KMID: 2471501
• DOI: http://doi.org/10.4055/jkoa.1974.9.2.165

Abstract

Bacterial suspensions of Staph. aureus, B. subtilis and the mixture of the two were added to the rabbit macrophage suspended in Cell Maintenance Fluid (CMF) and normal rabbit serum. After an incubation period of 30 and 60 minutes at 37℃ respectively, the bacteria-macrophage suspensions were investigated as regard to the percentage of macrophage with intracellular organisms and the percentage of intracellular organisms. Results of observation were summarized as follows: 1. In the Staph. aureus-macrophage suspension; The percentages of macrophage with intracellular organisms were 45.5% after 30 minutes. 68% after 60 minutes in normal rabbit serum, and 47% after 30 minutes, 80.5% after 60 minutes respectively. Percentages of intracellular organisms were 91% after 30 minutes. 97.5% after 60 minutes in normal rabbit serum. and 79.5% after 30 minutes. 89.5% after 60 minutes in CMF. 2. In the B. subtilis-macrophage suspension; The percentages of macrophage with intracellular organisms were 16% after 30 minutes, 26% after 60 minutes in normal rabbit serum, and 27% after 30 minutes, 44.5% after 60 minutes respectively. percentages of intracellular organisms were 82% after 30 minutes. 90% after 60 minutes in CMF. 3. In the bacterial mixture macrophage suspension; The percentages of macrophage with intracellular organisms were 12.5% after 30 minutes, 33% after 60 minutes in normal rabbit serum, and 16.5% after 30 minutes, 36% after 60 minutes in CMP respectively. The percentages of intracellular organisms, on the other hand, were 89% (Staph. aureas 77.5%, B. subtilis 11.5%) after 30 minutes. 93% after 60 minutes(S. aureus 83%, B. subtilis 10%) in normal rabbit serum, and 63% (S. aureus 57%, B. subtilis 6%) after 30 minutes. 80%(S. aureus 71%, B. subtilis 9%) after 60 minutes in CMF.

MeSH Terms

Hand
Macrophages*
Suspensions
Suspensions

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