J Korean Orthop Assoc.  1973 Jun;8(2):85-94. 10.4055/jkoa.1973.8.2.85.

In Vitro Observation of the Phagocytic Activities of Normal Rabbit Macrophages on the Microbial Cells of Staph. albus, Staph. aureus. Es. Coli and Ps. aeruginosa

• Lee EY

• KMID: 2463760
• DOI: http://doi.org/10.4055/jkoa.1973.8.2.85

Abstract

Bacterial suspensions of Staph. albus, Staph. aureus, Es. coli and Ps. aeruginosa were added into the rabbit macrophage suspension and placed in the cubator of 37℃. After the incubation of 30, 60 and 120 minutes, percentage of macrophage with intracellular organisms, percentage of intracellular organisms, and number of bacterial cells in the macrophages were followed by microscopy of Giemsa stained preperations. Results of observation were summarized as follows. :1. In the Staph. albus-macrophage suspension; In the suspension of cell-bacterial ratio (CBR) being 1:1 or less, percentages of macrophages with intracellular organisms were small upto 120 minutes of incubation. In the suspension of CBR being about 1:100, percentage of macrophages with intracellular organisms was 40% after 30 mintes, and more than 90% after 120 minutes. Percentage of intracellular organisms was 60-80% after 30 minutes and more than 90% after 120 minutes. The more the number of organisms were present, or the longer the time of incubation elaspsed the number of intracellular organisms were greater. 2. In the Staph. aureus-macrophage suspension; Percentage of macrophages with intracellular organisms was about 25% after 30 minutes and about 85% after 120 minutes in the suspension of CBR being 1:10. In the suspension of CBR being 1:100, percentage of macrophages with intracellular organisms was 45% after 30 minutes and more than 90% after 60 minutes. 55% to 90% of organisms were located intracellularly after 30minutes and the percentages increased until 60 minutes. After 120 minutes the percentages of intracellular organisms, in the suspensions of CBR being 1:1 or more, decreased considerablly. Compared to the Staph. albus-cell suspension, more organisms were found intracellularly. 3. In the Es. coli-macrophage suspension, In the suapension of CBR being 1:100 percentage of macrophages with intracellular organisms was about 70% after 30 minutes and 99% after 120 minutes. After 30 minutes 45-70% of organism were located intracellularly and 70%-80% after 60 minutes and the percentages continued increase in the suspensions of CBR being 1:1 or less, but decreased in the suspensions of CBR being 1:10 or more. Compared to the Staphylococcus-cell suspensions, number of organisms observed intracellularly were generally smaller. 4. In the Ps. aeruginosa-macrophage suspension; About 60% of macrophages were found haboring organisms in the suspension of CBR being 1:100 after 30 minutes and 85% after 120 minutes. After 30minutes about 90% of organisms were observed intracellularly in the suspension of CBR 1:10 or more and after 60 minutes the percentage decreased. Compared to the Es. coli-macrophage suspension, the number of intracellular organisms were about the same or even less.

MeSH Terms

Azure Stains
In Vitro Techniques*
Macrophages*
Mentha
Microscopy
Suspensions
Azure Stains
Suspensions