Nat Prod Sci.  2019 Dec;25(4):334-340. 10.20307/nps.2019.25.4.334.

Development and Validation of HPLC-PDA Method and Pattern Recognition Analyses Using Eight Marker Compounds for the Quality Control Between the Seeds of Cuscuta chinensis Lam. and Cuscuta japonica Choisy

• Nguyen DH
• Zhao BT
• Le DD
• Ma ES
• Min BS
• Woo MH

• KMID: 2468067
• DOI: http://doi.org/10.20307/nps.2019.25.4.334

Abstract

Cuscuta chinensis Lam. and Cuscuta japonica Choisy are parasitic plants. C. chinensis seeds were traditionally used for treatment of kidney and liver deficiencies. C. japonica seeds were used as tonic medicine to improve liver function and strengthen kidneys, treatment of high blood pressure, chronic diarrhea, and sore eyes. Cuscutae Semen are seeds of only C. chinensis in Korean Herbal Pharmacopoeia (K.H.P.). The developed HPLC-PDA method easily, accurately, and sensitively quantified using eight marker compounds [hyperoside (1), astragalin, (2), quercetin (3), kaempferol (4), chlorogenic acid (5), 3,4-di-O-caffeoylquinic acid (6), 1,5-di-O-caffeoylquinic acid (7), and 4,5-di-O-caffeoylquinic acid (8)]. In addition, the method may be used to distinguish seeds between C. chinensis Lam. and C. japonica Choisy. Furthermore, the result from the current study was applied to clarify samples between steam processed and unprocessed samples of C. chinensis by pattern analysis.

Keyword

Cuscuta chinensis; Cuscuta japonica; Flavonoids; Caffeoylquinic acid; HPLC-PDA

MeSH Terms

Chlorogenic Acid
Cuscuta*
Diarrhea
Flavonoids
Hypertension
Kidney
Liver
Methods*
Quality Control*
Quercetin
Semen
Steam
Chlorogenic Acid
Flavonoids
Quercetin
Steam

Figure

• 

  Fig. 1 The structures of marker compounds (1 – 8) of C. chinensis and C. japonica samples.

• 

  Fig. 2 The HPLC chromatograms of C. chinensis (A) and C. japonica (B) samples and the standard mixture (C). Hyperoside (1), astragalin (2), quercetin (3), kaempferol (4), chlorogenic acid (5), 3,4-di-O-caffeoylquinic acid (6), 1,5-di-O-caffeoylquinic acid (7), 4,5-di-O-caffeoylquinic acid (8), and an internal standard (IS, caffeic acid).

• 

  Fig. 3 The cluster analysis of C. chinensis and C. japonica seed samples using the contents of eight marker compounds (1 – 8). C. japonica seed samples (J07 – J12, A), C. chinensis steam processed (C01 – C03, B) and C. chinensis unprocessed seed samples (C04 – C06, C).

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