Int J Oral Biol.  2019 Sep;44(3):108-114. 10.11620/IJOB.2019.44.3.108.

Preparation and characterization of rutile phase TiOâ‚‚ nanoparticles and their cytocompatibility with oral cancer cells

  • 1Department of Pharmacology and Dental Therapeutics, College of Dentistry, Chosun University, Gwangju 61452, Republic of Korea.


In the present study, rutile phase titanium dioxide nanoparticles (R-TiOâ‚‚ NPs) were prepared by hydrolysis of titanium tetrachloride in an aqueous solution followed by calcination at 900℃. The composition of R-TiOâ‚‚ NPs was determined by the analysis of X-ray diffraction data, and the characteristic features of R-TiOâ‚‚ NPs such as the surface functional group, particle size, shape, surface topography, and morphological behavior were analyzed by Fourier-transform infrared spectroscopy, scanning electron microscopy and energy dispersive X-ray spectroscopy, transmission electron microscopy, dynamic light scattering, and zeta potential measurements. The average size of the prepared R-TiOâ‚‚ NPs was 76 nm, the surface area was 19 m²/g, zeta potential was −20.8 mV, and average hydrodynamic diameter in dimethyl sulfoxide (DMSO)-Hâ‚‚O solution was 550 nm. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and morphological observations revealed that R-TiOâ‚‚ NPs were cytocompatible with oral cancer cells, with no inhibition of cell growth and proliferation. This suggests the efficacy of R-TiOâ‚‚ NPs for the aesthetic white pigmentation of teeth.


Titanium dioxide nanoparticles; Rutile; Cytocompatibility; FaDu cells; YD-15 cells
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