J Adv Prosthodont.  2017 Dec;9(6):482-485. 10.4047/jap.2017.9.6.482.

Biofilm formation on denture base resin including ZnO, CaO, and TiOâ‚‚ nanoparticles

• Anwander M
• Rosentritt M
• Schneider-Feyrer S
• Hahnel S

• KMID: 2398054
• DOI: http://doi.org/10.4047/jap.2017.9.6.482

Abstract

PURPOSE
This laboratory study aimed to investigate the effect of doping an acrylic denture base resin material with nanoparticles of ZnO, CaO, and TiOâ‚‚ on biofilm formation.
MATERIALS AND METHODS
Standardized specimens of a commercially available cold-curing acrylic denture base resin material were doped with 0.1, 0.2, 0.4, or 0.8 wt% commercially available ZnO, CaO, and TiOâ‚‚ nanopowder. Energy dispersive X-ray spectroscopy (EDX) was used to identify the availability of the nanoparticles on the surface of the modified specimens. Surface roughness was determined by employing a profilometric approach; biofilm formation was simulated using a monospecies Candida albicans biofilm model and a multispecies biofilm model including C. albicans, Actinomyces naeslundii, and Streptococcus gordonii. Relative viable biomass was determined after 20 hours and 44 hours using a MTT-based approach.
RESULTS
No statistically significant disparities were identified among the various materials regarding surface roughness and relative viable biomass.
CONCLUSION
The results indicate that doping denture base resin materials with commercially available ZnO, CaO, or TiOâ‚‚ nanopowders do not inhibit biofilm formation on their surface. Further studies might address the impact of varying particle sizes as well as increasing the fraction of nanoparticles mixed into the acrylic resin matrix.

Keyword

Biofilm; Denture; Nanoparticles; PMMA; Candida albicans

MeSH Terms

Actinomyces
Biofilms*
Biomass
Candida albicans
Denture Bases*
Dentures*
Nanoparticles*
Particle Size
Polymethyl Methacrylate
Spectrometry, X-Ray Emission
Streptococcus gordonii
Polymethyl Methacrylate

Figure

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  Fig. 1 Surface roughness (Ra) of the various modified denture base resins and the unmodified control. Means and standard deviations are indicated.

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  Fig. 2 Relative absorbance values indicating the relative amount of adherent viable cells in the monospecies biofilm model. Means and standard deviations are indicated.

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  Fig. 3 Relative absorbance values indicating the relative amount of adherent viable cells in the multispecies biofilm model. Means and standard deviations are indicated.

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