J Lab Med Qual Assur.  2019 Sep;41(3):172-178. 10.15263/jlmqa.2019.41.3.172.

Comparison of Red Blood Cell, White Blood Cell and Differential Counts between UF-5000 System and Manual Method

• Koo MS
• Lim J
• Kim SY
• Koo SH
• Kwon GC

• KMID: 2460782
• DOI: http://doi.org/10.15263/jlmqa.2019.41.3.172

Abstract

BACKGROUND
Analysis of body fluids provides important information for assessing various medical conditions. We aimed to validate the analytical and diagnostic performance of the Sysmex UF-5000 (Sysmex, Japan) system for the analysis of different body fluids.
METHODS
Eighty body fluid samples were analyzed using the UF-5000 system in the body fluid mode and light microscopy. Body fluids included ascitic, pleural, and cerebrospinal fluid (CSF), as well as other fluid samples.
RESULTS
A comparison between the UF-5000 system and manual counting demonstrated good correlations with regard to red (r=0.6555) and white blood cell (r=0.9666) counts. The UF-5000 system also demonstrated good performance for differential cell counting (r=0.9028). CSF particularly showed a good correlation.
CONCLUSIONS
The use of the UF-5000 system for cell counting and differential analysis of body fluid samples might be an effective and automated alternative to chamber counting in laboratory routine analysis, thereby enhancing laboratory workflow and clinical effectiveness.

Keyword

Sysmex UF-5000; Automation; Laboratory; Body fluids

MeSH Terms

Automation
Body Fluids
Cell Count
Cerebrospinal Fluid
Erythrocytes*
Leukocytes*
Methods*
Microscopy
Treatment Outcome