Clin Exp Reprod Med.
2019 Sep;46(3):132-139. 10.5653/cerm.2019.00122.
Analysis of vitamin D-binding protein (VDBP) gene polymorphisms in Korean women with and without endometriosis
- Affiliations
-
- 1Department of Laboratory Medicine, Gyeongsang National University Hospital and Gyeongsang National University College of Medicine, Jinju, Korea.
- 2Institute of Health Sciences, Gyeongsang National University, Jinju, Korea. choiwj@gnu.ac.kr
- 3Biomedical Research Institute, Gyeongsang National University Hospital, Jinju, Korea.
- 4Department of Obstetrics and Gynecology, Gyeongsang National University Hospital, Jinju, Korea.
- 5Department of Obstetrics and Gynecology, Gyeongsang National University Changwon Hospital, Changwon, Korea.
- 6Department of Obstetrics and Gynecology, Gyeongsang National University Hospital and Gyeongsang National University College of Medicine, Jinju, Korea.
- KMID: 2458501
- DOI: http://doi.org/10.5653/cerm.2019.00122
Abstract
OBJECTIVE
Vitamin D-binding protein (VDBP) mediates various biological processes in humans. The goal of this study was to investigate whether VDBP gene polymorphisms could predispose Korean women to endometriosis.
METHODS
We prospectively enrolled women with endometriosis (n = 16) and healthy controls (n = 16). Total serum 25-hydroxyl vitamin D (25(OH)D) concentrations were measured using an Elecsys vitamin D total kit. Levels of bioavailable and free 25(OH)D were calculated. Concentrations of VDBP were measured using a vitamin D BP Quantikine ELISA kit. DNA was extracted using a DNeasy blood & tissue kit. Two single-nucleotide polymorphisms (SNPs; rs4588 and rs7041) in GC, the gene that codes for VDBP, were analyzed using a TaqMan SNP genotyping assay kit. The functional variant of VDBP was determined based on the results of the two SNPs.
RESULTS
Gravidity and parity were significantly lower in the endometriosis patients than in the control group, but serum CA-125 levels and the erythrocyte sedimentation rate were significantly higher. Total serum 25(OH)D levels in the endometriosis patients were significantly lower than in the control group. However, serum bioavailable 25(OH)D, free 25(OH)D, and VDBP levels did not differ significantly between the endometriosis and control groups. The genotypes and allele frequencies of GC were similar in both groups.
CONCLUSION
Korean women with endometriosis had lower total serum 25(OH)D concentrations than controls. Neither serum VDBP concentrations nor polymorphisms in the gene coding for VDBP were associated with endometriosis. Further studies are needed to investigate the pathophysiology and clinical implications of 25(OH)D and VDBP in endometriosis.
MeSH Terms
-
Biological Processes
Blood Sedimentation
Clinical Coding
DNA
Endometriosis*
Enzyme-Linked Immunosorbent Assay
Female
Gene Frequency
Genotype
Gravidity
Humans
Parity
Polymorphism, Genetic
Polymorphism, Single Nucleotide
Prospective Studies
Vitamin D
Vitamin D-Binding Protein*
Vitamins*
DNA
Vitamin D
Vitamin D-Binding Protein
Vitamins
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Differences in 25-hydroxy vitamin D and vitamin D-binding protein concentrations according to the severity of endometriosis
- Clinical Utility of Measurement of Vitamin D-Binding Protein and Calculation of Bioavailable Vitamin D in Assessment of Vitamin D Status
- A lack of association between vitamin D-binding protein and 25-hydroxyvitamin D concentrations in pediatric type 1 diabetes without microalbuminuria
- Correlational Analysis of Bone Health Status and Vitamin D-Related Biomarkers in Women Working in Agriculture
- CYP19 gene variant confers susceptibility to endometriosis-associated infertility in Chinese women
