Mycobiology.  2019 Jun;47(2):256-260. 10.1080/12298093.2019.1616377.

Neuraminidase Inhibitors from the Fruiting Body of Glaziella splendens

• Kim JY
• Woo EE
• Ha LS
• Ki DW
• Lee IK
• Yun BS

• KMID: 2455706
• DOI: http://doi.org/10.1080/12298093.2019.1616377

Abstract

Neuraminidase (NA) cleaves the glycosidic bond linkages of sialic acids to release the mature virions from infected cells and has been an attractive therapeutic target for anti-influenza agents. In our ongoing investigation of NA inhibitors in mushroom extracts, we found that the extract the fruiting body of Glaziella splendens potently inhibited neuraminidase. The fruiting bodies of G. splendens were extracted and partitioned successively with hexane, ethyl acetate, and butanol. The ethyl acetate soluble-layer was subjected to silica gel and Sephadex LH-20 column chromatographies, and MPLC to obtain five compounds (1-5). Their structures were determined by spectroscopic methods. NA inhibitory activity of these compounds was evaluated using NAs from recombinant rvH1N1, H3N2, and H5N1 influenza A viruses. One compound (1) was elucidated as a new azaphilone derivative, and four compounds (2-5) were identified as entonaemin A, comazaphilone D, rubiginosin A, and entonaemin B, respectively. Compounds 3 and 4 showed considerable inhibitory activity against three types of neuraminidases with the ICâ‚…â‚€ values of 30.9, 41.8, and 35.7"‰µM for 3 and 46.5, 50.4, and 29.9"‰µM for 4, respectively. This study reveals that the fruiting bodies of G. splendens possess azaphilone derivatives with the NA inhibitory activity. This is the first report on the isolation of neuraminidase inhibitors from the fruiting bodies of G. splendens.

Keyword

Azaphilone; Glaziella splendens; Neuraminidase inhibitor

MeSH Terms

Agaricales
Chromatography
Fruit*
Influenza A virus
N-Acetylneuraminic Acid
Neuraminidase*
Sialic Acids
Silica Gel
Virion
N-Acetylneuraminic Acid
Neuraminidase
Sialic Acids
Silica Gel