Korean J Parasitol.  2019 Jun;57(3):283-290. 10.3347/kjp.2019.57.3.283.

Development and Clinical Evaluation of a Rapid Diagnostic Test for Yellow Fever Non-Structural Protein 1

• Kim YH
• Kim TY
• Park JS
• Park JS
• Lee J
• Moon J
• Chong CK
• Junior IN
• Ferry FR
• Ahn HJ
• Bhatt L
• Nam HW

• KMID: 2453317
• DOI: http://doi.org/10.3347/kjp.2019.57.3.283

Abstract

A rapid diagnostic test (RDT) kit was developed to detect non-structural protein 1 (NS1) of yellow fever virus (YFV) using monoclonal antibody. NS1 protein was purified from the cultured YFV and used to immunize mice. Monoclonal antibody to NS1 was selected and conjugated with colloidal gold to produce the YFV NS1 RDT kit. The YFV RDTs were evaluated for sensitivity and specificity using positive and negative samples of monkeys from Brazil and negative human blood samples from Korea. Among monoclonal antibodies, clones 3A11 and 3B7 proved most sensitive, and used for YFV RDT kit. Diagnostic accuracy of YFV RDT was fairly high; Sensitivity was 0.0% and specificity was 100% against Dengue viruses type 2 and 3, Zika, Chikungunya and Mayaro viruses. This YFV RDT kit could be employed as a test of choice for point-of-care diagnosis and large scale surveys of YFV infection under clinical or field conditions in endemic areas and on the globe.

Keyword

Yellow fever; diagnostics; RDT; monoclonal antibody; NS1

MeSH Terms

Animals
Antibodies, Monoclonal
Brazil
Clone Cells
Dengue Virus
Diagnosis
Diagnostic Tests, Routine*
Gold Colloid
Haplorhini
Humans
Korea
Mice
Point-of-Care Systems
Sensitivity and Specificity
Yellow fever virus
Yellow Fever*
Antibodies, Monoclonal
Gold Colloid