Nat Prod Sci.  2019 Jun;25(2):172-180. 10.20307/nps.2019.25.2.172.

Thymol Rich Thymbra capitata Essential Oil Inhibits Quorum Sensing, Virulence and Biofilm Formation of Beta Lactamase Producing Pseudomonas aeruginosa

• Qaralleh H

• KMID: 2452788
• DOI: http://doi.org/10.20307/nps.2019.25.2.172

Abstract

Infections with Pseudomonas aeruginosa are difficult to treat not only because it is often associated with multidrug-resistant infections but also it is able to form biofilm. The aim of this study was to evaluate the antibiofilm and anti-Quorum Sensing (QS) activities of Thymbra capitata essential oils (EOs) against Beta Lactamase (BL) producing P. aeruginosa and the reference strain P. aeruginosa 10145. GC/MS analysis showed that thymol (23.25%) is the most dominant compound in T. capitata EOs. The MICs of T. capitata EOs against P. aeruginosa (BL) and P. aeruginosa 10145 were 1.11%. At sub MIC (0.041, 0.014 and 0.0046%), the EOs of T. capitata remarkably inhibited the biofilm formation of both strains tested and complete inhibition of the biofilm formation was reported at 0.041%. The EOs of T. capitata were found to inhibit the swarming motility, aggregation ability and hydrophobic ability of P. aeruginosa (BL) and P. aeruginosa 10145. Interestingly, the EOs of T. capitata reduce the production of three secreted virulence factors that regulated by QS system including pyocyanin, rhamnolipids and LasA protease. The potent antibiofilm and anti-QS activities of T. capitata EOs can propose it as a new antibacterial agent to control pseudomonas infections.

Keyword

Pseudomonas aeruginosa; virulence; quorum sensing; biofilm; Thymbra capitata; essential oils

MeSH Terms

beta-Lactamases*
Biofilms*
Oils, Volatile
Pseudomonas aeruginosa*
Pseudomonas Infections
Pseudomonas*
Pyocyanine
Quorum Sensing*
Thymol*
Virulence Factors
Virulence*
Oils, Volatile
Pyocyanine
Thymol
Virulence Factors
beta-Lactamases

Figure

• 

  Fig. 1 The inhibition effect of different concentrations (0.041, 0.014 and 0.0046%) of T. capitata EOs on the formation of biofilms of P. aeruginosa (BL) and P. aeruginosa 10145 using crystal violet assay.

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  Fig. 2 The effect of different concentrations (0.041 and 0.014%) of T. capitata EOs on swarming motility of P. aeruginosa (BL) and P. aeruginosa 10145. A: control, B: treated with 0.014% of T. capitata EOs, C: treated with 0.041% of T. capitata EOs.

• 

  Fig. 3 Effects of different concentrations (0.041, 0.014 and 0.0046%) of T. capitata EOs on the aggregation ability of P. aeruginosa (BL) and P. aeruginosa 10145.

• 

  Fig. 4 Effect of different concentrations (0.041, 0.014 and 0.0046%) of T. capitata EOs on the hydrophobicity of P. aeruginosa (BL) and P. aeruginosa 10145 using hydrocarbon separation ratio (FPc).

• 

  Fig. 5 The effect of different concentrations (0.041, 0.014 and 0.0046%) of T. capitata EOs on pyocyanin production in P. aeruginosa (BL) and P. aeruginosa 10145.

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  Fig. 6 The effect of different concentrations (0.041, 0.014 and 0.0046%) of T. capitata EOs on rhamnolipidproduction in P. aeruginosa (BL) and P. aeruginosa 10145.

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  Fig. 7 staphylolytic protease activity of P. aeruginosa (BL) and P. aeruginosa 10145 treated with different concentrations (0.041, 0.014 and 0.0046%) of T. capitata EOs.

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