Osong Public Health Res Perspect.  2019 Jun;10(3):170-176. 10.24171/j.phrp.2019.10.3.08.

Genetic Variability of Methicillin Resistant Staphylococcus Aureus Strains Isolated from Burns Patients

Affiliations
  • 1Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. gudarzim@yahoo.com
  • 2Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo, Japan.
  • 3Department of Virology, Pasteur Institute of Iran, Tehran, Iran.
  • 4School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Abstract


OBJECTIVES
Staphylococcus aureus is a nosocomial pathogen that provides a major challenge in the healthcare environment, especially in burns units where patients are particularly susceptible to infections. In this study, we sought to determine molecular types of S. aureus isolates collected from burns patients, based on staphylococcal protein A and coagulase gene polymorphisms.
METHODS
Antibiotic susceptibility testing of 89 S. aureus strains isolated from burn wounds of patients was assessed using the Kirby-Bauer disk diffusion method. Strains were characterized by spa typing, coa typing, and resistance and toxin gene profiling.
RESULTS
A total of 12 different spa types were identified with the majority being t790 (18%). Panton-Valentine leucocidin encoding genes were identified in spa types t044 (5.6%), t852 (2.2%) and t008 (2.2%). The most commonly detected antibiotic resistance gene was ant (4"²)-Ia (60.7%). Ten different coa types were detected and the majority of the tested isolates belonged to coa III (47.2%). All the high-level mupirocin-resistant and low-level mupirocin resistant strains belonged to coa type III.
CONCLUSION
The present study illustrated that despite the high frequency of coa III and spa t790 types, the genetic background of S. aureus strains in Iranian burns patients was diverse. The findings obtained are valuable in creating awareness of S. aureus infections within burns units.

Keyword

burn; coagulase; methicillin-resistant Staphylococcus aureus; minimum inhibitory concentration; Staphylococcus aureus
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