Korean J Vet Res.  2019 Jun;59(2):81-88. 10.14405/kjvr.2019.59.2.81.

Various macromolecules in in vitro growth medium influence growth, maturation, and parthenogenetic development of pig oocytes derived from small antral follicles

Affiliations
  • 1College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Korea. eslee@kangwon.ac.kr
  • 2Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, Korea.
  • 3Division of Applied Animal Science, Kangwon National University, Chuncheon 24341, Korea.

Abstract

This study was performed to examine the effects of various macromolecules in in vitro growth (IVG) media on the growth, maturation, and parthenogenesis (PA) of pig oocytes derived from small antral follicles (SAF). Immature oocytes were cultured for two days in IVG medium supplemented with 10% (v/v) fetal bovine serum (FBS), 10% (v/v) pig follicular fluid (PFF), 0.4% (w/v) bovine serum albumin (BSA), or 0.1% (w/v) polyvinyl alcohol (PVA) and then maintained for 44 h for maturation. After IVG, the mean diameters of the SAF treated with FBS, PVA, and no IVG-MAF (113.0-114.8 µm) were significantly larger than that of no IVG-SAF (111.8 µm). The proportion of metaphase II oocytes was higher in PFF (73.6%) than in BSA (43.5%) and PVA (53.7%) but similar to that in the FBS treatment (61.5%). FBS and PFF increased cumulus expansion significantly compared to PVA and BSA while the intraoocyte glutathione content was not influenced by the macromolecules. Blastocyst formation of PA oocytes treated with FBS (51.8%), PFF (50.4%), and PVA (45.2%) was significantly higher than that of the BSA-treated oocytes (20.6%). These results show that the PFF and FBS treatments during IVG improved the growth, maturation, and embryonic development of SAF.

Keyword

in vitro maturation; macromolecules; oocyte growth; small antral follicles

MeSH Terms

Blastocyst
Embryonic Development
Female
Follicular Fluid
Glutathione
In Vitro Techniques*
Metaphase
Oocytes*
Parthenogenesis
Polyvinyl Alcohol
Pregnancy
Serum Albumin, Bovine
Glutathione
Polyvinyl Alcohol
Serum Albumin, Bovine
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