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Oocyte maturation under a biophoton generator improves preimplantation development of pig embryos derived by parthenogenesis and somatic cell nuclear transfer

Lee J, Shin H, Lee W, Lee ST, Lee GS, Hyun SH, Lee E

This study was conducted to determine the effects of biophoton treatment during in vitro maturation (IVM) and/or in vitro culture (IVC) on oocyte maturation and embryonic development in pigs. An...
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Development of in vitro produced porcine embryos according to serum types as macromolecule

Son J, Malaweera , Lee E, Shin S, Cho J

This study was conducted to establish an in vitro maturation (IVM) system by selection of efficient porcine serum during porcine in vitro production. To investigate the efficient porcine serum (PS),...
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Various macromolecules in in vitro growth medium influence growth, maturation, and parthenogenetic development of pig oocytes derived from small antral follicles

Lee H, Lee Y, Lee J, Lee GS, Lee ST, Lee E

This study was performed to examine the effects of various macromolecules in in vitro growth (IVG) media on the growth, maturation, and parthenogenesis (PA) of pig oocytes derived from small...
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Identification of abnormal gene expression in bovine transgenic somatic cell nuclear transfer embryos

Cho J, Kang S, Lee BC

This study was conducted to investigate the expression of three genes related to early embryonic development in bovine transgenic cloned embryos. To accomplish this, development of bovine transgenic somatic cell...
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Rapamycin treatment during in vitro maturation of oocytes improves embryonic development after parthenogenesis and somatic cell nuclear transfer in pigs

Lee J, Park JI, Yun JI, Lee Y, Yong H, Lee ST, Park CK, Hyun SH, Lee GS, Lee E

This study was conducted to investigate the effects of rapamycin treatment during in vitro maturation (IVM) on oocyte maturation and embryonic development after parthenogenetic activation (PA) and somatic cell nuclear...
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Effect of IP3 and ryanodine treatments on the development of bovine parthenogenetic and reconstructed embryos

Ahn GJ, Lee BC, Hwang WS

  • KMID: 1103521
  • J Vet Sci.
  • 2001 Aug;2(2):131-137.
For parthenogenetic activation as a model system of nuclear transfer, microinjection and electroporation as activation treatments in bovine metaphase II oocytes were administered to each of three groups as follows:...
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Dynamic analysis of Ca2+ level during bovine oocytes maturation and early embryonic development

Liang SL, Zhao QJ, Li XC, Jin YP, Wang YP, Su XH, Guan WJ, Ma YH

Mammalian oocyte maturation and early embryo development processes are Ca(2+)-dependent. In this study, we used confocal microscopy to investigate the distribution pattern of Ca2+ and its dynamic changes in the...
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Enhancing effects of serum-rich and cytokine-supplemented culture conditions on developing blastocysts and deriving porcine parthenogenetic embryonic stem cells

Jung SK, Kim HJ, Kim CL, Lee JH, You JY, Lee ES, Lim JM, Yun SJ, Song JY, Cha SH

The present study was conducted to develop an effective method for establishment of porcine parthenogenetic embryonic stem cells (ppESCs) from parthenogenetically activated oocyte-derived blastocysts. The addition of 10% fetal bovine...
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