Korean J Vet Res.  2019 Jun;59(2):69-74. 10.14405/kjvr.2019.59.2.69.

Molecular characterization of Plasmodium juxtanucleare in Thai native fowls based on partial cytochrome C oxidase subunit I gene

• Pohuang T
• Junnu S

• KMID: 2451640
• DOI: http://doi.org/10.14405/kjvr.2019.59.2.69

Abstract

Avian malaria is one of the most important general blood parasites of poultry in Southeast Asia. Plasmodium (P.) juxtanucleare causes avian malaria in wild and domestic fowl. This study aimed to identify and characterize the Plasmodium species infecting in Thai native fowl. Blood samples were collected for microscopic examination, followed by detection of the Plasmodium cox I gene by using PCR. Five of the 10 sampled fowl had the desired 588 base pair amplicons. Sequence analysis of the five amplicons indicated that the nucleotide and amino acid sequences were homologous to each other and were closely related (100% identity) to a P. juxtanucleare strain isolated in Japan (AB250415). Furthermore, the phylogenetic tree of the cox I gene showed that the P. juxtanucleare in this study were grouped together and clustered with the Japan strain. The presence of P. juxtanucleare described in this study is the first report of P. juxtanucleare in the Thai native fowl of Thailand.

Keyword

fowl; cytochrome C oxidase subunit I; Plasmodium juxtanucleare

MeSH Terms

Amino Acid Sequence
Animals
Asia, Southeastern
Asian Continental Ancestry Group*
Base Pairing
Cytochromes c*
Cytochromes*
Electron Transport Complex IV*
Humans
Japan
Malaria, Avian
Parasites
Plasmodium*
Polymerase Chain Reaction
Poultry
Sequence Analysis
Thailand
Trees
Cytochromes
Cytochromes c
Electron Transport Complex IV