Ann Lab Med.  2019 Nov;39(6):580-583. 10.3343/alm.2019.39.6.580.

Fluoranthene-Induced Cytotoxicity and Direct Effect of Aryl Hydrocarbon Receptor Antagonist on Hematopoietic Stem Cell Differentiation

• Yang SH
• Kang MG
• Kim HR
• Lee YE
• Na BR
• Lee JH
• Shin JH
• Shin MG

• KMID: 2450955
• DOI: http://doi.org/10.3343/alm.2019.39.6.580

Abstract

No abstract available.

MeSH Terms

Hematopoietic Stem Cells*
Receptors, Aryl Hydrocarbon*
Receptors, Aryl Hydrocarbon

Figure

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  Fig. 1 Time- and dose-dependent effect of AhR antagonist treatment on HSC self-renewal proliferation in suspension cultures from autologous and allogeneic PB-HSC fractions. (A) Effect of AhR antagonist on the self-renewal of the autologous HSC fraction. SR1 and E03 resulted in the greatest increase in self-renewal proliferation in the autologous PB-HSC faction compared with other AhR antagonists. (B) Effect of AhR antagonist on the self-renewal of the allogeneic HSC fraction. UM and HIT resulted in the greatest increase in self-renewal proliferation in the allogeneic PB-HSC faction compared with other AhR antagonists.Abbreviations: UN, untreated; DMSO, dimethyl sulfoxide; HSC, hematopoietic stem cell; PB-HSC, peripheral blood-derived hematopoietic stem cell; AhR, aryl hydrocarbon receptor; UM, UM171; HIT, Hit 90186.

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  Fig. 2 Effect of the AhR antagonists on HSC differentiation using a colony forming unit (CFU) assay for PBSC. (A) Exposure of the PBSC fraction to FR induced remarkable suppression of CFC formation. (B) Treatment of the PB-HSC fraction with AhR antagonists (CH and D03) did not show a remarkable effect on HSC differentiation, whereas co-treatment of CH and D03 with FR restored cell number, as well as reconstructed cell morphology. Thus, AhR antagonists seem to have some restorative effect on PBSC following FR exposure.Abbreviations: CFC, colony-forming cell; DMSO, dimethyl sulfoxide; HSC, hematopoietic stem cell; PB-HSC, peripheral blood-derived hematopoietic stem cell; PBSC, peripheral blood-derived stem cell; AhR, aryl hydrocarbon receptor; FR, fluoranthene.

Reference

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