Tissue Eng Regen Med.  2019 Apr;16(2):131-139. 10.1007/s13770-018-0156-4.

Proliferation Profile of Uterine Endometrial Stromal Cells during In Vitro Culture with Gonadotropins: Recombinant versus Urinary Follicle Stimulating Hormone

• Kim YJ
• Kim YY
• Song DY
• Lee SH
• Park CW
• Kim H
• Ku SY

• KMID: 2449244
• DOI: http://doi.org/10.1007/s13770-018-0156-4

Abstract

BACKGROUND
Provision of optimal endometrial stromal cells is essential in uterine tissue engineering. Culture of these cells is significantly influenced by gonadotropin hormones. This investigation attempted to define the proliferation profiles of murine uterine endometrial stromal cells during in vitro culture with recombinant follicle stimulating hormone (rFSH), urinary follicle stimulating hormone (uFSH), and human chorionic gonadotropin (hCG).
METHODS
Murine uterine endometrial stromal cells were collected from 8-week-old mice and cultured in vitro up to 72 h, with rFSH, uFSH, or hCG. Cell cycles were analyzed by BrdU assay, and cyclin D1 expression was evaluated according to dose and duration of gonadotropin treatment.
RESULTS
BrdU assay showed a further inhibitory effect on murine uterine endometrial stromal cell proliferation when cultured with rFSH compared to uFSH, and a similar inhibitory proliferation profile when cultured with hCG at a specific range of concentrations. The expression of cyclin D1 of murine uterine endometrial stromal cells was down-regulated when cultured with rFSH, uFSH, or hCG, compared to control.
CONCLUSIONS
FSH may inhibit the proliferation of murine uterine endometrial stromal cells during in vitro culture. rFSH may have more significant inhibitory effects on the proliferation of endometrial stromal cells than uFSH. Establishing an optimal endocrine milieu is necessary using more advanced combination of female hormones for in vitro culture of this type of cells.

Keyword

Uterus; Endometrial stromal cells; In vitro culture

MeSH Terms

Animals
Bromodeoxyuridine
Cell Cycle
Chorionic Gonadotropin
Cyclin D1
Female
Follicle Stimulating Hormone*
Gonadotropins*
Humans
In Vitro Techniques*
Mice
Stromal Cells*
Tissue Engineering
Uterus
Bromodeoxyuridine
Chorionic Gonadotropin
Cyclin D1
Follicle Stimulating Hormone
Gonadotropins