Int Neurourol J.
2019 Feb;23(Suppl 1):S22-S31. 10.5213/inj.1938038.019.
Effects of Acute Exercise on Mitochondrial Function, Dynamics, and Mitophagy in Rat Cardiac and Skeletal Muscles
- Affiliations
-
- 1Department of Kinesiology, Inha University, Incheon, Korea. kwakhb@inha.ac.kr
- 2Department of Pharmacology and Medicinal Toxicology Research Center, Inha University School of Medicine, Incheon, Korea.
- 3Department of Sports & Outdoors, College of Health Industry, Eulji University, Seongnam, Korea.
- 4National Research Laboratory for Mitochondrial Signaling, Department of Physiology, Cardiovascular and Metabolic Disease Center, Inje University College of Medicine, Busan, Korea.
- 5Department of Leisure Sports, Seoil University, Seoul, Korea.
- KMID: 2448804
- DOI: http://doi.org/10.5213/inj.1938038.019
Abstract
- PURPOSE
This study aimed to investigate the effects of single-bout exercise on mitochondrial function, dynamics (fusion, fission), and mitophagy in cardiac and skeletal muscles.
METHODS
Fischer 344 rats (4 months old) were randomly divided into the control (CON) or acute exercise (EX) group (n=10 each). The rats performed a single bout of treadmill exercise for 60 minutes. Mitochondrial function (e.g., Oâ‚‚ respiration, Hâ‚‚Oâ‚‚ emission, Ca²âº retention capacity), mitochondrial fusion (e.g., Mfn1, Mfn2, Opa1), mitochondrial fission (e.g., Drp1, Fis1), and mitophagy (e.g., Parkin, Pink1, LC3II, Bnip3) were measured in permeabilized cardiac (e.g., left ventricle) and skeletal (e.g., soleus, white gastrocnemius) muscles.
RESULTS
Mitochondrial Oâ‚‚ respiration and Ca²âº retention capacity were significantly increased in all tissues of the EX group compared with the CON group. Mitochondrial Hâ‚‚Oâ‚‚ emissions showed tissue-specific results; the emissions showed no significant differences in the left ventricle or soleus (type I fibers) but was significantly increased in the white gastrocnemius (type II fibers) after acute exercise. Mitochondrial fusion and fission were not altered in any tissues of the EX group. Mitophagy showed tissue-specific differences: It was not changed in the left ventricle or white gastrocnemius, whereas Parkin and LC3II were significantly elevated in the soleus muscle.
CONCLUSIONS
A single bout of aerobic exercise may improve mitochondrial function (e.g., Oâ‚‚ respiration and Ca²âº retention capacity) in the heart and skeletal muscles without changes in mitochondrial dynamics or mitophagy.
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