Korean J Vet Res.  2019 Mar;59(1):43-45. 10.14405/kjvr.2019.59.1.43.

Conditions for the disinfectant efficacy test under subzero temperatures

• Chae WS
• Jeong W
• Lee HJ

• KMID: 2448110
• DOI: http://doi.org/10.14405/kjvr.2019.59.1.43

Abstract

To establish appropriate conditions for a disinfectant efficacy test at subzero temperatures, this study examined mixtures of frozen foot-and-mouth disease virus or avian influenza virus solutions and disinfectant diluents at −5℃ and monitored temperature and freezing status of an anti-freezing diluent (AFD, 15% ethanol + 30% propylene glycol + 55% distilled water) over time at various subzero temperatures. Viral solutions and disinfectant diluents froze before the mixtures reached −5℃, whereas the AFD was not frozen at −30℃. The times taken for the AFD to reach −10, −20, −30, and −40℃ from room temperature were 36, 39, 45, and 48 min, respectively.

Keyword

foot-and-mouth disease virus; avian influenza virus; disinfectant efficacy test; subzero temperature; anti-freezing diluent

MeSH Terms

Animals
Ethanol
Foot-and-Mouth Disease Virus
Freezing
Influenza in Birds
Propylene Glycol
Ethanol
Propylene Glycol