Nutr Res Pract.  2018 Oct;12(5):365-370. 10.4162/nrp.2018.12.5.365.

Antioxidative and antiproliferative activities of ethanol extracts from pigmented giant embryo rice (Oryza sativa L. cv. Keunnunjami) before and after germination

• Chung SI
• Lee SC
• Yi SJ
• Kang MY

• KMID: 2446995
• DOI: http://doi.org/10.4162/nrp.2018.12.5.365

Abstract

BACKGROUND/OBJECTIVES
Oxidative stress is a major cause of cancer. This study investigated the effects of the ethanol extracts from germinated and non-germinated Keunnunjami rice, a blackish-purple pigmented cultivar with giant embryo, on selected human cancer cell lines and on the antioxidant defense system of mice fed with a high-fat diet.
MATERIALS/METHODS
High fat-fed mice were orally administered with either distilled water (HF) or extracts (0.25%, w/w) from brown (B), germinated brown (GB), Keunnunjami (K), and germinated Keunnunjami (GK) rice.
RESULTS
In comparison with the brown rice extract, Keunnunjami extract showed higher anticancer effect against cervical and gastric cell lines but lower anticancer activity on liver and colon cancer cells. Mice from the HF group showed significantly higher lipid peroxidation and lower antioxidant enzyme activities than the control group. However, the oxidative stress induced by high-fat diet markedly decreased in B, GB, K, and GK groups as compared with the HF group.
CONCLUSIONS
Germination may be an effective method for improving the anticancer and antioxidative properties of Keunnunjami rice and extracts from germinated Keunnunjami rice may serve as a therapeutic agent against cervical and gastric cancers and oxidative damage.

Keyword

Rice; germination; cancer; antioxidant; nutritional and metabolic diseases

MeSH Terms

Animals
Cell Line
Colonic Neoplasms
Diet, High-Fat
Embryonic Structures*
Ethanol*
Germination*
Humans
Lipid Peroxidation
Liver
Methods
Mice
Nutritional and Metabolic Diseases
Oxidative Stress
Stomach Neoplasms
Water
Ethanol
Water

Reference

1. Han SJ, Kwon SW, Chu SH, Ryu SN. A new rice variety ‘Keunnunjami’, with high concentrations of cyanidin 3-glucoside and giant embryo. Korean J Breed Sci. 2012; 44:185–189.

2. Chung SI, Rico CW, Lee SC, Kang MY. Hypolipidemic and body fat-lowering effects of giant embryo brown rice (Seonong 17 and Keunnunjami) in high-fat-fed mice. Cereal Chem. 2014; 91:18–22.

3. Chung SI, Lo LM, Nam SJ, Jin X, Kang MY. Antioxidant capacity of giant embryo rice Seonong 17 and Keunnunjami. J Adv Agric Technol. 2016; 3:94–98.

4. Laokuldilok T, Shoemaker CF, Jongkaewwattana S, Tulyathan V. Antioxidants and antioxidant activity of several pigmented rice brans. J Agric Food Chem. 2011; 59:193–199.

5. Min B, McClung AM, Chen MH. Phytochemicals and antioxidant capacities in rice brans of different color. J Food Sci. 2011; 76:C117–C126.

6. Goufo P, Trindade H. Rice antioxidants: phenolic acids, flavonoids, anthocyanins, proanthocyanidins, tocopherols, tocotrienols, γ-oryzanol, and phytic acid. Food Sci Nutr. 2014; 2:75–104.

7. Oh CH, Oh SH. Effects of germinated brown rice extracts with enhanced levels of GABA on cancer cell proliferation and apoptosis. J Med Food. 2004; 7:19–23.

8. Zhang L, Hu P, Tang S, Zhao H, Wu D. Comparative studies on major nutritional components of rice with a giant embryo and a normal embryo. J Food Biochem. 2005; 29:653–661.

9. Wu F, Yang N, Touré A, Jin Z, Xu X. Germinated brown rice and its role in human health. Crit Rev Food Sci Nutr. 2013; 53:451–463.

10. Cho DH, Lim ST. Germinated brown rice and its bio-functional compounds. Food Chem. 2016; 196:259–271.

11. Moongngarm A, Saetung N. Comparison of chemical compositions and bioactive compounds of germinated rough rice and brown rice. Food Chem. 2010; 122:782–788.

12. Patil SB, Khan MK. Germinated brown rice as a value added rice product: a review. J Food Sci Technol. 2011; 48:661–667.

13. Ng LT, Huang SH, Chen YT, Su CH. Changes of tocopherols, tocotrienols, γ-oryzanol, and γ-aminobutyric acid levels in the germinated brown rice of pigmented and nonpigmented cultivars. J Agric Food Chem. 2013; 61:12604–12611.

14. Hübner F, Arendt EK. Germination of cereal grains as a way to improve the nutritional value: a review. Crit Rev Food Sci Nutr. 2013; 53:853–861.

15. Nelson K, Stojanovska L, Vasiljevic T, Mathai M. Germinated grains: a superior whole grain functional food? Can J Physiol Pharmacol. 2013; 91:429–441.

16. Wu F, Chen H, Yang N, Wang J, Duan X, Jin Z, Xu X. Effect on germination time on physicochemical properties of brown rice flour and starch from different rice cultivars. J Cereal Sci. 2013; 58:263–271.

17. Chung SI, Rico CW, Kang MY. Comparative study on the hypoglycemic and antioxidative effects of fermented paste (doenjang) prepared from soybean and brown rice mixed with rice bran or red ginseng marc in mice fed with high fat diet. Nutrients. 2014; 6:4610–4624.

18. Lo LM, Kang MY, Yi SJ, Chung SI. Dietary supplementation of germinated pigmented rice (Oryza sativa L.) lowers dyslipidemia risk in ovariectomized Sprague-Dawley rats. Food Nutr Res. 2016; 60:30092.

19. Chakuton K, Puangpronpitag D, Nakornriab M. Phytochemical content and antioxidant activity of colored and non-colored Thai rice cultivars. Asian J Plant Sci. 2012; 11:285–293.

20. Jeng TL, Shih YJ, Ho PT, Lai CC, Lin YW, Wang CS, Sung JM. γ-Oryzanol, tocol and mineral compositions in different grain fractions of giant embryo rice mutants. J Sci Food Agric. 2012; 92:1468–1474.

21. Konwachara T, Ahromrit A. Effect of cooking on functional properties of germinated black glutinous rice (KKU-URL012). Songklanakarin J Sci Technol. 2014; 36:283–290.

22. Velioglu YS, Mazza G, Gao L, Oomah BD. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem. 1998; 46:4113–4117.

23. Reeves PG, Nielsen FH, Fahey GC Jr. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr. 1993; 123:1939–1951.

24. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95:351–358.

25. Islam MA, Becerra JX. Analysis of chemical components involved in germination process of rice variety Jhapra. J Sci Res. 2012; 4:251–262.

26. Norhaizan ME, Ng SK, Norashareena MS, Abdah MA. Antioxidant and cytotoxicity effect of rice bran phytic acid as an anticancer agent on ovarian, breast and liver cancer cell lines. Malays J Nutr. 2011; 17:367–375.

27. Watanabe M, Maemura K, Oki K, Shiraishi N, Shibayama Y, Katsu K. Gamma-aminobutyric acid (GABA) and cell proliferation: focus on cancer cells. Histol Histopathol. 2006; 21:1135–1141.

28. Yang CS, Suh N. Cancer prevention by different forms of tocopherols. Top Curr Chem. 2013; 329:21–33.

29. Zhou Y, Zheng J, Li Y, Xu DP, Li S, Chen YM, Li HB. Natural polyphenols for prevention and treatment of cancer. Nutrients. 2016; 8:E515.

30. Chen PN, Chu SC, Chiou HL, Chiang CL, Yang SF, Hsieh YS. Cyanidin 3-glucoside and peonidin 3-glucoside inhibit tumor cell growth and induce apoptosis in vitro and suppress tumor growth in vivo. Nutr Cancer. 2005; 53:232–243.

31. Cho E, Chung EY, Jang HY, Hong OY, Chae HS, Jeong YJ, Kim SY, Kim BS, Yoo DJ, Kim JS, Park KH. Anti-cancer effect of cyanidin-3-glucoside from mulberry via caspase-3 cleavage and DNA fragmentation in vitro and in vivo. Anticancer Agents Med Chem. 2017; 17:1519–1525.

32. Hui C, Bin Y, Xiaoping Y, Long Y, Chunye C, Mantian M, Wenhua L. Anticancer activities of an anthocyanin-rich extract from black rice against breast cancer cells in vitro and in vivo. Nutr Cancer. 2010; 62:1128–1136.

33. Pintha K, Yodkeeree S, Pitchakarn P, Limtrakul P. Anti-invasive activity against cancer cells of phytochemicals in red jasmine rice (Oryza sativa L.). Asian Pac J Cancer Prev. 2014; 15:4601–4607.

34. Kim HY, Lee SH, Hwang IG, Kim TM, Park DS, Kim JH, Kim DJ, Lee J, Jeong HS. Antioxidant activity and anticancer effects of rough rice (Oryza sativa L.) by germination periods. J Korean Soc Food Sci Nutr. 2012; 41:14–19.

35. Ibrahim W, Lee US, Yeh CC, Szabo J, Bruckner G, Chow CK. Oxidative stress and antioxidant status in mouse liver: effects of dietary lipid, vitamin E and iron. J Nutr. 1997; 127:1401–1406.

36. Reiter RJ, Tan DX, Burkhardt S. Reactive oxygen and nitrogen species and cellular and organismal decline: amelioration with melatonin. Mech Ageing Dev. 2002; 123:1007–1019.

37. Ng CJ, Shih DM, Hama SY, Villa N, Navab M, Reddy ST. The paraoxonase gene family and atherosclerosis. Free Radic Biol Med. 2005; 38:153–163.

38. Lee YR, Kim CE, Kang MY, Nam SH. Cholesterol-lowering and antioxidant status-improving efficacy of germinated giant embryonic rice (Oryza sativa L.) in high cholesterol-fed rats. Ann Nutr Metab. 2007; 51:519–526.

39. Maksup S, Pongpakpian S, Roytrakul S, Cha-Um S, Supaibulwatana K. Comparative proteomics and protein profile related to phenolic compounds and antioxidant activity in germinated Oryza sativa ‘KDML105’ and Thai brown rice ‘Mali Daeng’ for better nutritional value. J Sci Food Agric. 2018; 98:566–573.

40. Scalbert A, Johnson IT, Saltmarsh M. Polyphenols: antioxidants and beyond. Am J Clin Nutr. 2005; 81:215S–217S.

41. Serafini M, Morabito G. The role of polyphenols in the modulation of plasma Non-Enzymatic Antioxidant Capacity (NEAC). Int J Vitam Nutr Res. 2012; 82:228–232.