J Nutr Health.  2019 Aug;52(4):399-407. 10.4163/jnh.2019.52.4.399.

Physicochemical properties of dried Saururus chinensis and the antioxidative activities of water and 70% ethanol extracts

• Kang MH
• An SM
• Kim DH

• KMID: 2455854
• DOI: http://doi.org/10.4163/jnh.2019.52.4.399

Abstract

PURPOSE
This study was conducted to evaluate the physicochemical properties of different batches of Saururus chinensis using different roasting temperature that were dried at different using different roasting temperatures and their were determined the antioxidative activities of water and 70% ethanol extracts.
METHODS
Extracts were examined for the total phenolic acid content, the and flavonoids contents and the antioxidant properties, including DPPH radical scavenging activity, ABTs scavenging activity and, the reducing power.
RESULTS
Moisture content was significantly higher in the LSC and the crude ash content was significantly higher in the HSC. The crude protein content was higher in the LSC (although not significantly), and the crude fat and carbohydrate contents were higher in the HSC (although not significantly). The total phenolic content was lower in the samples extracted with water, but there was no significant difference. However, the extracts extracted with 70% ethanol at a high drying temperature were significantly higher. The low temperature and high drying temperature batches of Saururus chinensis were significantly higher in the samples extracted with 70% ethanol than those extracted with 70% ethanol. The total phenolic acid content, the total flavonoid content and the electron donating ability were highest in the ethanol extract of Saururuschinensis treated at a high temperature. However, the ABTs radical activity was highest in the water extracted, high-temperature treated Saururuschinensis. The 70% ethanol extract of high temperature roasted Saururuschinensis had the highest antioxidative activities of all the Saururuschinensis batches.
CONCLUSION
The total phenolic acid contents, total flavonoid contents, electron donating activity and reducing power activity were highest in all the 70% ethanol extraction batches of the high-temperature treated samples.

Keyword

Saururus chinensis; DPPH radicals; physicochemical; antioxidative; ABTs

MeSH Terms

Ethanol*
Flavonoids
Phenol
Saururaceae*
Water*
Ethanol
Flavonoids
Phenol
Water

Reference

1. Chung BS, Shin MG. Dictionary of Korean folk medicine. Seoul: Young Lim Publishing;1990.

2. Koh MS. Antimicrobial activity of Saururus chinensis Baill extract. J Korean Soc Food Sci Nutr. 2004; 33(7):1098–1105.

3. Cho YJ. Antioxidant, angiotensinconverting enzyme and xanthin oxidase inhibitory activity of extracts from Saururus chinensis leaves by ultrafine grinding. Korean J Food Preserv. 2014; 21(1):75–81.

4. Lee YN. Flora of Korea. Seoul: Kyohak Publishing;2002.

5. Hong JH, Lee WY. Quality characteristics of osmotic dehydrated sweet pumpkin by different drying methods. J Korean Soc Food Sci Nutr. 2004; 33(9):1573–1579.

6. Kim BM. Foodstuffs storage study. Seoul: Jin-ro Moonwhasa;2005.

7. Holdsworth SD. Dehydration of food products A review. Int J Food Sci Technol. 1971; 6(4):331–370.

8. Kim KO, Kim JM, Lee YC. Effects of convection oven dehydration conditions on the physicochemical and sensory properties of ginkgo nut powder. Korean J Food Sci Technol. 2003; 35(3):393–398.

9. Lee HG, Lee KS, Park KH, Lee SH, Choe EO. The quality properties of dried carrots as affected by blanching and drying methods during storage. Korean J Food Sci Technol. 2003; 35(6):1086–1092.

10. Akyel C, Bosisio RG, Chahine R, Bose TK. Measurement of the complex permittivity of food products during microwave power heating cycles. J Microw Power. 1983; 18(4):355–365.

11. Seo HS, Chung BH, Cho YG. Antioxidant and anticancer effects of agrimony (Agrimonia pilosa L.) and Chinese Lizardtail (Saururus chinensis Baill). Korean J Med Crop Sci. 2008; 16(3):139–143.

12. Kim IJ, Kim MJ, Nam SY, Yun T, Kim HS, Hong SS, et al. Contents of quercetin glycoside and lignans according to the cultivated years and plant parts in Saururus chinensis Baill. Korean J Pharmacogn. 2006; 37(1):42–47.

13. Park SJ, Yoo HJ, Choi HS, Seo BY, Yang SH, Kim YH, et al. Effects of Saururus chinensis Baill extracts on anticancer activity and cadmium induced cytotoxicity. Korean J Orient Prev Med. 2004; 8:81–98.

14. Ju JC, Shin JH, Lee SJ, Cho HS, Sung NJ. Antioxidative activity of hot water extracts from medicinal plants. J Korean Soc Food Sci Nutr. 2006; 35(1):7–14.

15. Association of Official Analytical Chemists (US). Official methods of analysis. 15th edition. Washington, D.C.: Association of Official Analytical Chemists;1990.

16. Lee SJ, Choi BB, Lee SW, Han MR, Lee JW. Food analysis easy to understand. Goyang: PowerBook;2014.

17. Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol. 1999; 299:152–178.

18. Masaki H, Sakaki S, Atsumi T, Sakurai H. Active-oxygen scavenging activity of plant extracts. Biol Pharm Bull. 1995; 18(1):162–166.

19. Brand-Williams W, Cuvelier ME, Berset C. Use of free radical method to evaluate antioxidant activity. Lebensm Wiss Technol. 1995; 28(1):25–30.

20. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999; 26(9-10):1231–1237.

21. Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974; 47(3):469–474.

22. Yen GC, Duh PD. Scavenging effect of methanolic extracts of peanut hulls on free-radical and active oxygen-species. J Agric Food Chem. 1994; 42(3):629–632.

23. National Institute of Agricultural Sciences (KR). Explanation on food composition [Internet]. Wanju: National Institute of Agricultural Sciences;2018. cited 2018 Nov 01. Available from: http://koreanfood.rda.go.kr.

24. Hwang JB, Yang MO, Shin HK. Survey for approximate composition and mineral content of medicinal herbs. Korean J Food Sci Technol. 1997; 29(4):671–679.

25. Park SJ, Lee JS, Hoe YH, Moon EY, Kang MH. Physiology activity of barley leaf using different drying methods. J Korean Soc Food Sci Nutr. 2008; 37(12):1627–1631.

26. Choi YH, Kim SE, Huh J, Han YH, Lee MJ. Antibacterial and antioxidative activity of roasted coffee and red ginseng mixture extracts. J Korean Soc Food Sci Nutr. 2012; 41(3):320–326.

27. Kim SM, Kim DY, Park HR, Seo JH, Yeom BM, Jin Y, et al. Screening the antioxidantcomponents and antioxidant activitiy of extracts derived from five varieties of edible spring flowers. Korean J Food Sci Technol. 2014; 46(1):13–18.

28. Kang JR, Kang MJ, Choi MH, Byun HU, Shin JH. Physicochemical characteristics of ethanol extract from Artemisia Argyi H. Using different preparation methods. J Life Sci. 2017; 27(1):23–31.

29. Park KD. Antioxidative and proliferative effects of follicle dermal papilla cell of extracts from Saururus chinensis and Phyllostachys Folium [dissertation]. Kwangju: Chosun University;2016.

30. Kim SK, Ban SY, Kim JS, Chung SK. Change of antioxidant activity and antioxidant compounds in Saururus chinensis by extraction conditions. J Appl Biol Chem. 2005; 48(1):89–92.

31. Hertog MG, Hollman PC, Katan MB. Content of potentially anticarcinogenic flavonoids of 28 vegetables and 9 fruits commonly consumed in the Netherlands. J Agric Food Chem. 1992; 40(12):2379–2383.

32. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 1999; 64(4):555–559.

33. Kim NY, Kim YK, Bae KJ, Choi JH, Moon JH, Park GH, et al. Free radical scavenging effect and extraction condition of ethanol extracts and fractions of wild grape seed (Vitis coignetiea). J Korean Soc Food Sci Nutr. 2005; 34(6):755–758.

34. Kang KM, Lee SH. Effects of extraction methods on the antioxidative activity of Artemisia sp. J Korean Soc Food Sci Nutr. 2013; 42(8):1249–1254.

35. Lee CY, Kim KM, Son HS. Optimal extraction conditions to produce rosemary extracts with higher phenolic content and antioxidant activity. Korean J Food Sci Technol. 2013; 45(4):501–507.

36. Jo YH, Seo GU, Yuk HG, Lee SC. Antioxidant and tyrosinase inhibitory activities of methanol extracts from Magnolia denudata and Magnolia denudata var. purpurascens flowers. Food Res Int. 2012; 47(2):197–200.

37. Okawa M, Kinjo J, Nohara T, Ono M. DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity of flavonoids obtained from some medicinal plants. Biol Pharm Bull. 2001; 24(10):1202–1205.

38. Oh YJ, Seo HR, Choi YM, Jung DS. Evaluation of antioxidant activity of the extracts from the aerial parts of Cnidium officinale Makino. Korean J Med Crop Sci. 2010; 18(6):373–378.

39. Kim DH. Antioxidant activity and nitric oxide production inhibitory activity of steaming Gastrodia elata Blume [dissertation]. Jeonju: Chonbuk National University;2014.

40. Jegadeeswari P, Daffodil ED, Mohan VR. Quantification of total phenolics, flavonoid and in vitro antioxidant activity of Aristolochia bracteata retz. Int J Pharm Pharm Sci. 2014; 6(1):747–752.

41. Jeong HM, Kim YS, Ahn SJ, Auh MS, Ahn JB, Kim KY. Effects of Zizyphus jujuba var. boeunesis extracts on the growth of intestinal microflora and its antioxidant activities. J Korean Soc Food Sci Nutr. 2011; 40(4):500–508.

42. Fukai T, Ushio-Fukai M. Superoxide dismutases: role in redox signaling, vascular function, and diseases. Antioxid Redox Signal. 2011; 15(6):1583–1606.

43. Hong SM, An SM, Jeong BN, Kang MH. Comparative studies of the in vitro antioxidant properties of 80% ethanol or water extracts of Formica rufa L. Gastrodia elata Blume, Cnidium officinale Makino and their's mixture. Korean J Food Cookery Sci. 2018; 34(1):27–33.

44. Oyaizu M. Studies on products of browning reaction: antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr Diet. 1986; 44(6):307–315.

45. Turkmen N, Sari F, Velioglu YS. Effects of extraction solvents on concentration and antioxidant activity of black and black mate tea polyphenols determined by ferrous tartrate and Folin-Ciocalteu methods. Food Chem. 2006; 99(4):835–841.

46. Jo KS, Kim HK, Ha JH, Park MH, Shin HS. Flavor compounds and storage stability of essential oil from garlic distillation. Korean J Food Sci Technol. 2006; 22(7):840–845.

47. Berlett BS, Stadtman ER. Protein oxidation in aging, disease, and oxidative stress. J Biol Chem. 1997; 272(33):20313–20316.

48. Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med. 1996; 20(7):933–956.